Antimicrobial Photodynamic Inactivation: An Alternative for Group B Vaginal Colonization in a Murine Experimental Model.

Background: , referred to as Group B (GBS), is a prominent bacterium causing life-threatening neonatal infections. Although antibiotics are efficient against GBS, growing antibiotic resistance forces the search for alternative treatments and/or prevention approaches. Antimicrobial photodynamic inactivation (aPDI) appears to be a potent alternative non-antibiotic strategy against GBS.

Optimization of Biofilm Culture in a Continuous Flow System for Photoinactivation Studies.

is a relevant cause of neonatal mortality. It can be transferred to infants via the vaginal tract and cause meningitis, pneumonia, arthritis, or sepsis, among other diseases. The cause of therapy ineffectiveness and infection recurrence is the growth of bacteria as biofilms.

Antimicrobial Photodynamic Inactivation Affects the Antibiotic Susceptibility of spp. Clinical Isolates in Biofilm and Planktonic Cultures.

and are opportunistic pathogens that can cause a vast variety of nosocomial infections. Moreover, belongs to the group of ESKAPE microbes, which are the main cause of hospital-acquired infections and are especially difficult to treat because of their resistance to many antibiotics. Antimicrobial photodynamic inactivation (aPDI) represents an alternative to overcome multidrug resistance problems.

Factors Determining the Susceptibility of Bacteria to Antibacterial Photodynamic Inactivation.

Photodynamic inactivation of microorganisms (aPDI) is an excellent method to destroy antibiotic-resistant microbial isolates. The use of an exogenous photosensitizer or irradiation of microbial cells already equipped with endogenous photosensitizers makes aPDI a convenient tool for treating the infections whenever technical light delivery is possible. Currently, aPDI research carried out on a vast repertoire of depending on the photosensitizer used, the target microorganism, and the light delivery system shows efficacy mostly on models.

Increased photoinactivation stress tolerance of Streptococcus agalactiae upon consecutive sublethal phototreatments.

Streptococcus agalactiae (Group B Streptococcus, GBS) is a common commensal bacterium in adults but remains a leading source of invasive infections in newborns, pregnant women, and the elderly, and more recently, causes an increased incidence of invasive disease in nonpregnant adults. Reduced penicillin susceptibility and emerging resistance to non-β-lactams pose challenges for the development and implementation of novel, nonantimicrobial strategies to reduce the burden of GBS infections. Antimicrobial photodynamic inactivation (aPDI) via the production of singlet oxygen or other reactive oxygen species leads to the successful eradication of pathogenic bacteria, affecting numerous cellular targets of microbial pathogens and indicating a low risk of resistance development.

An extended logistic model of photodynamic inactivation for various levels of irradiance using the example of Streptococcus agalactiae.

Irradiance is an important factor influencing the acceleration of microorganism mortality in photodynamic inactivation (PDI) processes. Experimental observations of PDI processes indicate that the greater the irradiation power is, the faster the decrease in the population size of microorganisms. However, commonly used mathematical models of PDI processes usually refer only to specific values of irradiance without taking into account the influence of change in irradiance on the dynamic properties of inactivation.

Development of Staphylococcus aureus tolerance to antimicrobial photodynamic inactivation and antimicrobial blue light upon sub-lethal treatment.

Antimicrobial photodynamic inactivation (aPDI) and antimicrobial blue light (aBL) are considered low-risk treatments for the development of bacterial resistance and/or tolerance due to their multitargeted modes of action. In this study, we assessed the development of Staphylococcus aureus tolerance to these phototreatments. Reference S.

Photoinactivation of ESKAPE pathogens: overview of novel therapeutic strategy.

The emergence of antimicrobial drug resistance requires development of alternative therapeutic options. Multidrug-resistant strains of Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Enterobacter spp.