Publications by authors named "Michaela Pribikova"

Article Synopsis
  • The cytokine TNF can lead to a type of cell death influenced by RIPK1, but this can be suppressed by two proteins, TANK and AZI2, which help regulate TBK1 kinase activation.
  • Mice lacking both TANK and AZI2 experience severe health issues like multi-organ inflammation and early death, which can be mitigated by disabling TNFR1 or using a modified RIPK1.
  • TANK and AZI2 work together in the TNF receptor signaling process, binding to different components at distinct times to maintain TBK1 activity and protect against excessive inflammation.
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Mature T cells are selected for recognizing self-antigens with low to intermediate affinity in the thymus. Recently, the relative differences in self-reactivity among individual T-cell clones were appreciated as important factors regulating their fate and immune response, but the role of self-reactivity in T-cell biology is incompletely understood. We addressed the role of self-reactivity in T-cell diversity by generating an atlas of mouse peripheral CD8 T cells, which revealed two unconventional populations of antigen-inexperienced T cells.

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Interleukin-17A (IL-17A) is a key mediator of protective immunity to yeast and bacterial infections but also drives the pathogenesis of several autoimmune diseases, such as psoriasis or psoriatic arthritis. Here we show that the tetra-transmembrane protein CMTM4 is a subunit of the IL-17 receptor (IL-17R). CMTM4 constitutively associated with IL-17R subunit C to mediate its stability, glycosylation and plasma membrane localization.

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Ag-inexperienced memory-like T (AIMT) cells are functionally unique T cells, representing one of the two largest subsets of murine CD8 T cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack of data from feral mice, and an unclear relationship between AIMT cells formation during aging represent major barriers for better understanding of their biology. We performed a thorough characterization of AIMT cells from mice of different genetic background, age, and hygienic status by flow cytometry and multiomics approaches, including analyses of gene expression, TCR repertoire, and microbial colonization.

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Article Synopsis
  • IL-17 plays a dual role in the immune system, offering protection against fungi and bacteria while also contributing to autoimmune diseases.
  • Researchers used a new mass spectrometry technique to analyze the IL-17 receptor (IL-17R) complex and discovered the linear ubiquitin chain assembly complex (LUBAC) as a key signaling component.
  • A crucial negative feedback loop involving the kinases TBK1 and IKKε was identified, which helps to terminate IL-17 signaling by modifying the adaptor ACT1 and releasing the ubiquitin ligase TRAF6, with NEMO having a unique role in negatively regulating this pathway.
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It is well established that lymphopenia induces the formation of the memory-phenotype T cells without the exposure to foreign antigens. More recently, the memory-phenotype antigen-inexperienced memory T cells were described in lymphoreplete mice and called virtual memory T cells. In this review, we compare multiple aspects of the biology of lymphopenia-induced memory T cells and virtual memory T cells, including cytokine requirements, the role of T-cell receptor specificity in the differentiation process, gene expression signature, and the immune response.

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Virtual memory T cells are foreign antigen-inexperienced T cells that have acquired memory-like phenotype and constitute 10-20% of all peripheral CD8 T cells in mice. Their origin, biological roles, and relationship to naïve and foreign antigen-experienced memory T cells are incompletely understood. By analyzing T-cell receptor repertoires and using retrogenic monoclonal T-cell populations, we demonstrate that the virtual memory T-cell formation is a so far unappreciated cell fate decision checkpoint.

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