Screening bisphenols in complex samples via a planar Arxula adeninivorans bioluminescence bioassay.

The Arxula yeast bisphenol screen (A-YBS) utilizes the bioluminescent Arxula adeninivorans yeast-based reporter cells for tailored analysis of bisphenols, one of the major endocrine-disrupting compound groups. For the first time, this bioreporter has been applied on the high-performance thin-layer chromatography (HPTLC) adsorbent surface to develop a respective planar bioluminescence bioassay (pA-YBS). The goal was to combine the advantages of HPTLC with a more selective bioassay detection for bisphenols.

Development of a new particulate 4-μm adsorbent layer for ultrathin-layer chromatography (miniaturized chromatogram).

The performance of particulate layers of a smaller particle size than given HPTLC plates was investigated. However, reducing the particle size and thus interporous space, the capillary flow is substantially reduced. For the first time, optimizations led to the successful use of a layer made of mainly 4-μm irregular silica gel particles as stationary phase in planar chromatography.