Evidence that dissociation of Spt16 from transcribed genes is partially dependent on RNA Polymerase II termination.

FACT (FAcilitates Chromatin Transactions) is a highly conserved histone chaperone complex in eukaryotic cells that can interact and manipulate nucleosomes in order to promote a variety of DNA-based processes and to maintain the integrity of chromatin throughout the genome. Whereas key features of the physical interactions that occur between FACT and nucleosomes have been elucidated in recent years, less is known regarding FACT functional dynamics . Using the system, we now provide evidence that at least at some genes dissociation of the FACT subunit Spt16 from their 3' ends is partially dependent on RNA Polymerase II (Pol II) termination.