Folate metabolism in plants: an Arabidopsis homolog of the mammalian mitochondrial folate transporter mediates folate import into chloroplasts.

The distribution of folates in plant cells suggests a complex traffic of the vitamin between the organelles and the cytosol. The Arabidopsis thaliana protein AtFOLT1 encoded by the At5g66380 gene is the closest homolog of the mitochondrial folate transporters (MFTs) characterized in mammalian cells. AtFOLT1 belongs to the mitochondrial carrier family, but GFP-tagging experiments and Western blot analyses indicated that it is targeted to the envelope of chloroplasts.

The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gbeta gamma binding site.

G-protein-coupled receptor kinase 2 (GRK2) is activated by free Gbetagamma subunits. A Gbetagamma binding site of GRK2 is localized in the carboxyl-terminal pleckstrin homology domain. This Gbetagamma binding site of GRK2 also regulates Gbetagamma-stimulated signaling by sequestering free Gbetagamma subunits.

Functional importance of nucleotide identities within the pea atp9 mitochondrial promoter sequence.

Sequences ranging from nucleotide positions -14 to +4 relative to the transcription start site constitute an in vitro functional pea atp9 promoter. A comparison of respective sequence segments surrounding 11 unambiguously identified transcription initiation sites of various dicotyledoneous plant species revealed the highest level of evolutionary fidelity of nucleotide identities within the conserved nonanucleotide motif (CNM), suggesting their importance for promoter function. Using a mitochondrial in vitro transcription system, a detailed analysis by site-directed mutagenesis now reveals that the alteration of nucleotides -6 to -2 and +1 within the CNM indeed reduces promoter activity by more than 80%.