Detergent-Resistant Membranes in Chloroplasts and Mitochondria of the Halophyte under Salt Stress.

Halophytes represent important models for studying the key mechanisms of salt tolerance. One approach to the development of new knowledge of salt tolerance is to study the properties of detergent-resistant membranes (DRMs). In this work, the lipid profiles of DRMs of chloroplasts and mitochondria of euhalophyte Willd, before and after their exposure to shock concentrations of NaCl, have been investigated.

Tonoplast of Beta vulgaris L. contains detergent-resistant membrane microdomains.

The experiments conducted on tonoplast of Beta vulgaris L. roots were performed to identify detergent-resistant lipid-protein microdomains (DRMs, interpreted as lipid rafts).The presence of DRMs can be found when dynamic clustering of sphingolipids, sterols, saturated fatty acids is registered, and the insolubility of these microdomains in nonionic detergents at low temperatures is proven.