Stress During Development of Experimental Endometriosis Influences Nerve Growth and Disease Progression.

Purpose: We have previously shown that stress prior to induction worsens clinical presentation and inflammatory parameters in a rat model of endometriosis. This study was designed to examine whether stress during the development of endometriosis can affect the growth of endometriotic implants through nerve growth and immune alterations.

Methods: Endometriosis was surgically induced in female Sprague-Dawley rats by suturing uterine horn implants onto the small intestine mesentery.

Microarray analysis of gene expression in granulosal cells from persistent follicles in cattle.

Granulosal cells form highly specialized membrane connections with the oocyte and each other, allowing the passage of regulatory molecules and metabolites between cells. Gene expression changes in granulosal cells may adversely affect oocyte competence resulting in early embryonic loss. The present study was conducted to analyze global gene expression profiles in granulosal cells from persistent ovarian follicles in cows.

Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.

Objective: To validate a rat model of endometriosis using complimentary DNA (cDNA) microarrays by identifying common gene expression patterns between experimental and natural disease.

Design: Autotransplantation rat model.

Setting: Medical school department.

Changes of maternal transcripts in oocytes from persistent follicles in cattle.

A high incidence of early embryonic loss is associated with prolonged dominance of follicles. The objective of the present experiment was to determine if persistence of a follicle resulted in alterations in mRNA expression of important genes in the oocyte. Cows were assigned to four groups: growing follicles on day 6 (G0h) or day 8 (G48h) and persistent follicles on day 13 (P0h) or day 15 (P48h) of the estrous cycle (estrus = day 0).

Concurrent ganirelix and follitropin beta therapy is an effective and safe regimen for ovulation induction in women with polycystic ovary syndrome.

Objective: To evaluate controlled ovarian stimulation cycles using the GnRH antagonist ganirelix in combination with the recombinant FSH, follitropin-beta, in women with polycystic ovary syndrome (PCOS).

Design: Prospective, nonrandomized clinical study.

Setting: Hospital-based infertility practice.