Hybrid spatial-temporal Mueller matrix imaging spectropolarimeter for high throughput plant phenotyping.

Many correlations exist between spectral reflectance or transmission with various phenotypic responses from plants. Of interest to us are metabolic characteristics, namely, how the various polarimetric components of plants may correlate to underlying environmental, metabolic, and genotypic differences among different varieties within a given species, as conducted during large field experimental trials. In this paper, we overview a portable Mueller matrix imaging spectropolarimeter, optimized for field use, by combining a temporal and spatial modulation scheme.

Multistatic fiber-based system for measuring the Mueller matrix bidirectional reflectance distribution function.

Bidirectionality effects can be a significant confounding factor when measuring hyperspectral reflectance data. The bidirectional reflectance distribution function (BRDF) can effectively characterize the reflectivity of surfaces to correct remote sensing measurements. However, measuring BRDFs can be time-consuming, especially when collecting Mueller matrix BRDF (mmBRDF) measurements of a surface via conventional goniometric techniques.

Flexible sensor patch for continuous carbon dioxide monitoring.

Monitoring and measurement of carbon dioxide (CO) is critical for many fields. The gold standard CO sensor, the Severinghaus electrode, has remained unchanged for decades. In recent years, many other CO sensor formats, such as detection based upon pH-sensitive dyes, have been demonstrated, opening the door for relatively simple optical detection schemes.

Practical spectral photography II: snapshot spectral imaging using linear retarders and microgrid polarization cameras.

Despite recent advances, customized multispectral cameras can be challenging or costly to deploy in some use cases. Complexities span electronic synchronization, multi-camera calibration, parallax and spatial co-registration, and data acquisition from multiple cameras, all of which can hamper their ease of use. This paper discusses a generalized procedure for multispectral sensing using a pixelated polarization camera and anisotropic polymer film retarders to create multivariate optical filters.

Computer vision for detecting field-evolved lepidopteran resistance to Bt maize.

Background: Resistance evolution of lepidopteran pests to Bacillus thuringiensis (Bt) toxins produced in maize and cotton is a significant issue worldwide. Effective toxin stewardship requires reliable detection of field-evolved resistance to enable the implementation of mitigation strategies. Currently, visual estimates of maize injury are used to document changing susceptibility.

Quantification of gray mold infection in lettuce using a bispectral imaging system under laboratory conditions.

Gray mold disease caused by the fungus damages many crop hosts worldwide and is responsible for heavy economic losses. Early diagnosis and detection of the disease would allow for more effective crop management practices to prevent outbreaks in field or greenhouse settings. Furthermore, having a simple, non-invasive way to quantify the extent of gray mold disease is important for plant pathologists interested in measuring infection rates.

Internal defect scanning of sweetpotatoes using interactance spectroscopy.

While standard visible-light imaging offers a fast and inexpensive means of quality analysis of horticultural products, it is generally limited to measuring superficial (surface) defects. Using light at longer (near-infrared) or shorter (X-ray) wavelengths enables the detection of superficial tissue bruising and density defects, respectively; however, it does not enable the optical absorption and scattering properties of sub-dermal tissue to be quantified. This paper applies visible and near-infrared interactance spectroscopy to detect internal necrosis in sweetpotatoes and develops a Zemax scattering simulation that models the measured optical signatures for both healthy and necrotic tissue.

Dual-beam potassium Voigt filter for atomic line imaging.

Spectrally narrowband imaging in remote sensing applications can be advantageous for detecting atomic emission features. This is especially useful in detecting specific constituents within rocket plumes, which are challenging to discern from naturally occurring sunglints. In this paper, we demonstrate a dual-beam technique, implemented with a Wollaston prism, for calibrating a Voigt magneto-optical filter for a linear polarizer's finite extinction ratio, as well as optical misalignment between the linear polarizers' transmission axes.

Optical crosstalk and off-axis modeling of an intrinsic coincident polarimeter.

Polarimeters have broad applications in remote sensing, astronomy, and biomedical imaging to measure the emitted, reflected, or transmitted state of polarization. An intrinsic coincident (IC) full-Stokes polarimeter was previously demonstrated by our group, in a free space configuration, by using stain-aligned polymer-based organic photovoltaics. To minimize the model's complexity, these were tilted to avoid crosstalk from back-reflections.

Microbolometer with a multi-aperture polymer thin-film array for neural-network-basedtarget identification.

Infrared imaging spectrometers are frequently used for detecting chemicals at standoff distances. Cost, size, and sensitivity are common tradeoffs in this regime, particularly when deploying infrared imaging arrays. In this work, we develop and characterize an infrared snapshot computational imaging spectrometer that leverages a multi-aperture filtered design.

Snapshot channeled imaging spectrometer using geometric phase holograms.

In this paper, we present the design and experimental demonstration of a snapshot imaging spectrometer based on channeled imaging spectrometry (CIS) and channeled imaging polarimetry (CIP). Using a geometric phase microlens array (GPMLA) with multiple focal lengths, the proposed spectrometer selects wavelength components within its designed operating waveband of 450-700 nm. Compared to other snapshot spectral imagers, its key components are especially suitable for roll-to-roll (R2R) rapid fabrication, which gives the spectrometer potential for low-cost mass production.

Imaging linear and circular polarization features in leaves with complete Mueller matrix polarimetry.

Spectropolarimetry of intact plant leaves allows to probe the molecular architecture of vegetation photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological information. In addition to the molecular signals due to the photosynthetic machinery, the cell structure and its arrangement within a leaf can create and modify polarization signals. Using Mueller matrix polarimetry with rotating retarder modulation, we have visualized spatial variations in polarization in transmission around the chlorophyll a absorbance band from 650 nm to 710 nm.

Mueller matrix polarimetry on plasma sprayed thermal barrier coatings for porosity measurement.

Yttria-stabilized zirconia (YSZ) is the most widely used material for thermal plasma sprayed thermal barrier coatings (TBCs) used to protect gas turbine engine parts in demanding operation environments. The superior material properties of YSZ coatings are related to their internal porosity level. By quantifying the porosity level, tighter control on the spraying process can be achieved to produce reliable coatings.

Spatially heterodyned snapshot imaging spectrometer.

Snapshot hyperspectral imaging Fourier transform (SHIFT) spectrometers are a promising technology in optical detection and target identification. For any imaging spectrometer, spatial, spectral, and temporal resolution, along with form factor, power consumption, and computational complexity are often the design considerations for a desired application. Motivated by the need for high spectral resolution systems, capable of real-time implementation, we demonstrate improvements to the spectral resolution and computation trade-space.

Achromatic Wollaston prism beam splitter using polarization gratings.

We describe a method to achromatize a Wollaston prism beam splitter by combining it with a polarization grating. The advantage of this technique, compared to refractive methods of correction, is that only one type of birefringent crystal is needed. Additionally, the assembly can be made thinner while remaining achromatized.

Imaging of in vitro parenteral drug precipitation.

Solid particulate matter introduced into the bloodstream as a result of parenteral drug administration can produce serious pathological conditions. Particulate matter that cannot be eliminated by pre-infusion filtration is often the result of drug precipitation that occurs when certain parenteral formulations are mixed with blood. A new device is designed to model the mixing of drug formulations with flowing blood utilizing a uniquely designed flow cell and a CCD camera to view the formulation as it is mixed with a blood surrogate in real time.

Neural network calibration of a snapshot birefringent Fourier transform spectrometer with periodic phase errors.

Systematic phase errors in Fourier transform spectroscopy can severely degrade the calculated spectra. Compensation of these errors is typically accomplished using post-processing techniques, such as Fourier deconvolution, linear unmixing, or iterative solvers. This results in increased computational complexity when reconstructing and calibrating many parallel interference patterns.

Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.

Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.

Athermalized channeled spectropolarimetry using a biaxial potassium titanyl phosphate crystal.

Channeled spectropolarimeters measure the polarization state of light as a function of wavelength. Typically, a channeled spectropolarimeter uses high-order retarders made of uniaxial crystal to amplitude modulate the measured spectrum with the Stokes polarization information. A primary limitation of these instruments is the thermal variability of the retarders, which necessitates frequent system recalibration.

On the exploitation of mid-infrared iridescence of plumage for remote classification of nocturnal migrating birds.

A challenging task in ornithology lies in identifying high-altitude nocturnal migrating bird species and genders. While the current approaches including radar, lunar obscuration, and single-band thermal imaging provide means of detection, a more detailed spectral or polarimetric analysis of light has the potential for retrieval of additional information whereby the species and sex could be determined. In this paper, we explore remote classification opportunities provided by iridescent features within feathers in the mid-infrared region.

Spatial heterodyne interferometry with polarization gratings.

The implementation of a polarization-based spatial heterodyne interferometer (SHI) is described. While a conventional SHI uses a Michelson interferometer and diffraction gratings, our SHI exploits mechanically robust Wollaston prisms and polarization gratings. A theoretical model for the polarization SHI is provided and validated with data from our proof of concept experiments.