Publications by authors named "Michael Simurdiak"
Article Synopsis
- Spectinabilin is a rare metabolite that belongs to a group called nitrophenyl-substituted polyketides.
- The researchers successfully cloned and expressed the spectinabilin gene cluster from the bacterium Streptomyces spectabilis.
- Interestingly, this gene cluster is more closely related to another gene cluster for aureothin than to the previously known spectinabilin cluster from Streptomyces orinoci, and they regulate their functions differently despite their similarities.
Rieske oxygenases catalyze a wide variety of important oxidation reactions. Here we report the characterization of a novel Rieske N-oxygenase, aminopyrrolnitrin oxygenase (PrnD) that catalyzes the unusual oxidation of an arylamine to an arylnitro group. PrnD from Pseudomonas fluorescens Pf5 was functionally expressed in Escherichia coli, and the activity of the purified PrnD was reconstituted, which required in vitro assembly of the Rieske iron-sulfur cluster into the protein and the presence of NADPH, FMN, and an E.
View Article and Find Full Text PDFA xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.
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