Quantitative PCR (qPCR), loop-mediated amplification (LAMP), and lateral flow strip-based recombinase polymerase amplification (RPA-LFS) assays were assessed for early detection of , the global causal agent of potato and tomato late blight, on passive wind-powered spore traps known as Spornados. Spore traps were deployed in potato and tomato fields during the 2018, 2019, and 2020 growing seasons in the provinces of Alberta, British Columbia, Manitoba, Prince Edward Island, and Ontario. All assays used DNA extracts from Spornado cassette membranes targeting the nuclear ribosomal internal transcribed spacer.
View Article and Find Full Text PDFMedEdPORTAL is a unique web-based peer-reviewed publication venue for clinical health educators sponsored by the Association of American Medical Colleges (AAMC). The open exchange of educational resources promotes professional collaboration across health professions. In 2008, the American Dental Education Association (ADEA) collaborated with AAMC to allow dental educators to use the platform to publish dental curriculum resources.
View Article and Find Full Text PDFIn September 2011, the Association of American Medical Colleges released the results of a survey conducted in 2010 on simulation activities at its member medical schools and teaching hospitals. In this commentary, we offer a synthesis of data and conclude that (1) simulation is used broadly at Association of American Medical Colleges member institutions, for many types of learners, including other health care professionals; (2) it addresses core training competencies and has many educational purposes; (3) its use in learner assessment is more prevalent at medical schools but is still significant at teaching hospitals; and (4) it requires a considerable investment of money, space, personnel, and time. These data confirm general perceptions about the state of simulation in North America for physician training.
View Article and Find Full Text PDFJ Pediatr Orthop B
September 2010
Is the articular cartilage of the immature ankle joint damaged by tibial lengthening? Sixteen immature rabbits underwent a 30% diaphyseal lengthening by tibial callotasis. Damage was assessed by scanning electronic microscopy and histomorphometry at the completion of distraction and after an additional 5 weeks. Despite joint contracture, little damage in the articular cartilage was observed in contrast to the knee joint.
View Article and Find Full Text PDFJ Pediatr Orthop B
November 2007
Limb lengthening by tibial callotasis is usually performed in the metaphysis but may cause growth inhibition. Is diaphyseal lengthening more advantageous? Sixteen immature rabbits underwent 30% diaphyseal lengthening by tibial callotasis. The tibial length was measured on radiographs at the end of the distraction period and after an additional 5 weeks.
View Article and Find Full Text PDFJ Pediatr Orthop B
September 2007
Bone-screw loosening in monolateral external fixators is a significant problem. This study classifies the radiographic appearance of the bone-screw interface and predicts which screws will become loose and those that will remain solidly fixed to bone. Five radiographic features were identified at the bone-screw interface.
View Article and Find Full Text PDFThe Vilarrubias method of limb lengthening is a percutaneous procedure with soft tissue releases. This reduces soft tissue tension and prevents joint contractures during longer lengthenings. This method has been used for 99 lower limb lengthenings.
View Article and Find Full Text PDFWe studied the influence of different parameters of bone quality on the fixation strength of bicondylar tibial plateau fractures and examined the relationship between these parameters. Bone quality was measured in the plateau of 16 cadaveric tibias using three modalities: dual-energy X-ray absorptiometry (DXA), peripheral quantitative computer tomography (pQCT), and spectral analysis of digitized radiographs (SADR). The tibias were divided into two groups by the median bone mineral density (BMD) and randomized to receive either dual plating or external fixator for the stabilization of a standardized bicondylar tibial fracture.
View Article and Find Full Text PDFSuccessful limb lengthening requires serial radiological evaluation of the progression of healing of the regenerate bone. However, there is no radiographic classification system that shows how the regenerate should progress during treatment in adults. The study aimed to address this need.
View Article and Find Full Text PDFHybrid external fixators have become more popular over recent years, and although biomechanical tests show varying results from paper to paper, a consistent finding is shear motion occurring at the fracture/osteotomy site during loading. This can be reduced to some extent by frame configuration, and with the use of olive stop wires, but both of these have limitations. We have investigated the use of threaded fine wires in a circular hybrid fixator.
View Article and Find Full Text PDFAlthough adequate reduction and stable fixation have been recognized to be the prime goals in the treatment of displaced tibial plateau fractures, the optimal fixation technique remains controversial. The lack of a reliable model and a standard methodology contribute to this situation. The purpose of this study is to develop an experimental model of a tibial plateau fracture and a testing methodology that reproduces the failure mode commonly seen in the clinical setting.
View Article and Find Full Text PDFBackground: Both clinical and radiographic techniques have been used to evaluate foot and ankle position and orientation. Clinical measurements have been shown to be less reliable than radiographic measurements. Because midfoot equinus deformity may coexist with ankle joint equinus deformity, a radiographic method of separating and measuring these two components is desirable.
View Article and Find Full Text PDFClin Biomech (Bristol)
January 2005
Background: Although clear differences in fracture site displacement have previously been demonstrated between transverse and oblique fracture models stabilised by an asymmetrical method, the direction of the obliquity has not been examined biomechanically.
Methods: Eight Sawbones tibiae were cut to represent oblique fractures: four ran from antero-inferior to postero-superior and four from antero-superior to postero-inferior. These were fixed with a Sheffield Ring Fixator and cyclically loaded in axial and off-axis compression.
Clin Biomech (Bristol)
August 2004
Background: Distraction-resisting force is generated in the soft tissues and callus during limb lengthening. Monitoring this force may offer a method of studying the behaviour of soft tissue and detecting the distraction osteogenesis related problems, and help to prevent complications. Changes in the post distraction period have not been previously investigated and there are no reports on the contribution of gastrocnemius to the distraction-resisting force.
View Article and Find Full Text PDFThe in vivo passive knee joint reaction force was measured in a rabbit model of tibial diaphyseal lengthening. This was based on the assumption that limb lengthening creates soft tissue tension that compresses the joint surface and generates the joint contact force. A measurement method was developed that involved the distraction of the joint and the determination of the distraction force that just separates the joint surfaces.
View Article and Find Full Text PDFClin Orthop Relat Res
October 2003
Quadricepsplasty has been described by Thompson and Judet to improve flexion in severely ankylosed knees. Judet's technique has potential advantages because it is less damaging to the quadriceps mechanism and addresses the problem of external fixator pin site tethering on the lateral side of the thigh. The outcome of Judet's quadricepsplasty was assessed in 10 consecutive patients who were treated with external fixation either as a primary treatment (three patients) or as a secondary treatment for nonunion or malunion (seven patients) in a limb reconstruction unit.
View Article and Find Full Text PDFClin Orthop Relat Res
October 2003
The authors evaluated the effect of the foot on the loading axis of the lower limb measured from radiographs in 30 pediatric patients. Deviation at the knee was calculated for the hip-ankle (traditional) and the hip-foot lines (heel lined up with a metal wire). A trigonometric model of the limb loading axis was developed with predicted mechanical axis deviations at the knee.
View Article and Find Full Text PDFClin Biomech (Bristol)
November 2003
Objective: To compare the mechanical strength of fixation of bicondylar tibial plateau fractures using internal and external fixation techniques.
Design: A randomised laboratory testing of fixation methods currently in clinical use.
Background: To reduce the high complication rate associated with the double plating, many surgeons have started to use less invasive techniques.
Fine-wire external fixation is accepted as a minimally invasive technique, which can provide better outcomes than traditional open methods in the management of complex fractures of the tibial plateau. Available fixators vary in their biomechanical stability, and we believe that a stable beam-loading system is essential for consistently good outcomes. We assessed, prospectively, the clinical, radiological and general health status of 20 of 21 consecutive patients with complex fractures of the tibial plateau who had been treated using a standard protocol, with percutaneous screw fixation and a neutralisation concept with a fine wire beam-loading fixator allowing early weight-bearing.
View Article and Find Full Text PDFWhere length discrepancy occurs in the skeletally mature lower leg, there may be a benefit in using the technique of tibial shortening. Existing techniques have not been widely adopted and are associated with a high incidence of soft tissue complications. We describe a technique of progressive shortening, which we believe is a safe method, having adapted this from techniques for dealing with traumatic bone loss.
View Article and Find Full Text PDFObjective: To investigate the outcome (clinical, radiographic, and general health status) of the surgical treatment of displaced bicondylar tibial plateau fractures (OTA-41C) in patients >60 years old.
Design: Prospective cohort study.
Setting: Limb reconstruction service, university teaching hospital.
J Bone Joint Surg Br
March 2003
We have reviewed, retrospectively, all children with a lower limb deformity who underwent an acute correction and lengthening with a monolateral fixator between 1987 and 1996. The patients were all under the age of 19 years and had a minimum follow-up of eight months after removal of the fixator. A total of 41 children had 57 corrections and lengthening.
View Article and Find Full Text PDFClinical experience and published studies suggest that oblique fractures of the tibia are associated with delayed healing and non-union. Experimental studies have attributed this to increased shear at the fracture site. We have adopted the practice of using supplementary olive wires to reduce shear when using circular fixation for these fractures.
View Article and Find Full Text PDFClin Biomech (Bristol)
February 2003
Objectives: To determine how the different approaches of constructing a hybrid external skeletal fixator affect the mechanical environment at the fracture site.
Design: A comparative biomechanical analysis of external fixators that represent a spectrum of designs from unilateral to circular fixators.
Background: The most pertinent parameter that affects fracture healing is the inter-fragmentary displacement.
A retrospective study of 32 patients who underwent tibial lengthening was performed in order to establish the need for distal tibio-fibular fixation. In 16 patients stabilization of the inferior tibio-fibular joint was carried out and in the other 16 no stabilization was performed. Three established and one new radiographic index of the tibio-fibular relationship at the ankle were used to assess proximal fibular migration.
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