Rapid oxidative release of fungal mannan for detection by immunoassay.

Detection of fungal cells in infected tissue by procedures such as potassium hydroxide (KOH) microscopy and histopathology are well-established methods in medical mycology. However, microscopy requires skilled personnel, specialized equipment, and may take considerable time to a result. An alternative approach is immunoassay for detection of fungal mannans in tissue as a biomarker for the presence of fungal cells.

Multi-platform Approach for Microbial Biomarker Identification Using as a Model.

The identification of microbial biomarkers is critical for the diagnosis of a disease early during infection. However, the identification of reliable biomarkers is often hampered by a low concentration of microbes or biomarkers within host fluids or tissues. We have outlined a multi-platform strategy to assess microbial biomarkers that can be consistently detected in host samples, using , the causative agent of Lyme disease, as an example.