FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver.

Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24 progenitors are less mature and have greater proliferative capacity than CD71/CD24. We present protocols for isolation of CD71/CD24 progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment.