Publications by authors named "Michael J Goblirsch"
Article Synopsis
- Chronic exposure to fungicides like pyraclostrobin negatively affects honey bee cell viability and mitochondrial function, particularly with longer exposure times.
- Short-term exposure to pyraclostrobin decreased oxygen consumption rates, indicating respiratory stress, while boscalid showed no significant effects.
- This study highlights the potential of using honey bee cell lines to evaluate the molecular impacts of environmental toxins on pollinator health.
The honey bee (Apis mellifera) is commonly infected by multiple viruses. We developed an experimental system for the study of such mixed viral infections in newly emerged honey bees and in the cell line AmE-711, derived from honey bee embryos. When inoculating a mixture of iflavirids [sacbrood bee virus (SBV), deformed wing virus (DWV)] and dicistrovirids [Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV)] in both live bee and cell culture assays, IAPV replicated to higher levels than other viruses despite the fact that SBV was the major component of the inoculum mixture.
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- A controlled in vitro culture system for honey bee cells has been developed to study the effects of pathogens, pesticides, and nutritional deficiencies on honey bees.
- The system involves using honey bee eggs during their development, allowing scientists to establish primary cultures and later create a continuous cell line (AmE-711) that is primarily composed of fibroblast-type cells.
- This cell line has been characterized and shows promise for future research in areas like honey bee development, genetics, and toxicology.
Article Synopsis
- Exploring effective methods for eliminating bed bugs involves balancing total insect kill with cost and energy efficiency.
- Whole-room heat treatments, applying high temperatures to a space, are a common approach, but the precise temperature and time needed for effectiveness are unclear.
- Experiments revealed that adult bed bugs require 48.3 °C for lethal heat exposure, while eggs need 54.8 °C, with adults surviving around 94.8 minutes at 45 °C compared to eggs surviving longer at both 45 °C and 48 °C, highlighting the need for further research into bed bug heat resistance.
2-Methoxyestradiol (2ME(2)), a physiologic metabolite of 17beta-estradiol (estrogen), has emerged as a promising cancer therapy because of its potent growth-inhibitory and proapoptotic effects on both endothelial and tumor cells. 2ME(2) also suppresses osteoclast differentiation and induces apoptosis of mature osteoclasts, and has been shown to effectively repress bone loss in an animal model of postmenopausal osteoporosis. Given these observations, we have examined whether 2ME(2) could effectively target metastasis to bone, osteolytic tumors, and soft tissue tumors.
View Article and Find Full Text PDFSkeletal metastases are a major source of morbidity for cancer patients. The purpose of this study was to evaluate the effects of megavoltage irradiation and antiangiogenic therapy on metastatic bone cancer. A tumor xenograft model was prepared in C3H/Scid mice using 4T1 murine breast carcinoma cells.
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- Primary and metastatic bone cancers are challenging to treat, necessitating innovative strategies to enhance patient outcomes and longevity.
- The study explored using osteoclast lineage cells as a gene delivery system targeting bone cancers, employing a specific enzyme/prodrug setup that showed significant tumor cell destruction without harming osteoclasts.
- Results demonstrated substantial reductions in tumor size in treated mice, indicating the potential of this approach for effective bone cancer therapy while preserving the osteoclast population.
Bone cancer pain is a devastating manifestation of metastatic cancer. Unfortunately, current therapies can be ineffective, and when they are effective, the duration of the patient's survival typically exceeds the duration of pain relief. New, mechanistically based therapies are desperately needed.
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