Publications by authors named "Michael J Bullen"
Regulatory elements (REs) consist of enhancers and promoters that occupy a significant portion of the noncoding genome and control gene expression programs either in or in Putative REs have been identified largely based on their regulatory features (co-occupancy of ESC-specific transcription factors, enhancer histone marks, and DNase hypersensitivity) in mouse embryonic stem cells (mESCs). However, less has been established regarding their regulatory functions in their native context. We deployed and regulatory elements scanning through saturating mutagenesis and sequencing (ctSCAN-SMS) to target elements within the ∼12-kb -region (REs; CREs) of the gene locus, as well as genome-wide 2,613 high-confidence REs (TREs), in mESCs.
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- The study explores how embryonic stem cells (ESC) maintain their ability to self-renew and remain pluripotent through regulatory networks involving transcription factors and epigenetic regulators.
- Researchers used a CRISPR-Cas9 screening method to identify two epigenetic regulators, TAF5L and TAF6L, that are crucial for the maintenance of mouse ESC (mESC) states.
- TAF5L and TAF6L were found to activate key genes like c-Myc and Oct4, using mechanisms such as H3K9ac deposition, influencing the MYC regulatory network essential for the self-renewal of mESCs.