Publications by authors named "Michael Francis Dutton"
The contamination of cow's milk at the farm level with aflatoxin M1 was investigated in South Africa. Samples of feeds, forage, maize and milk were taken at nine dairy farms, and at the same time samples of the processed milk (retail milk) were collected from the respective dairies to which the farms delivered their milk. The feeds were analysed for aflatoxin B1 and the milk samples for aflatoxin M1 using high performance liquid chromatography (HPLC) and fluorescence detection.
View Article and Find Full Text PDFTwenty-one rice samples from field (ten), store (six) and market (five) from the traditional rice-growing areas of Niger State, Nigeria were analysed for aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisin B(1) (FB(1)) and B(2) (FB(2)), and patulin (PAT) by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) respectively. T-2 toxin was determined using TLC only. AFs were detected in all samples, at total AF concentrations of 28-372 μg/kg.
View Article and Find Full Text PDFArticle Synopsis
- Lymphocytes from healthy humans and pigs were tested for their viability after being exposed to the mycotoxins fumonisin B1 (FB1) and ochratoxin A (OTA), known for their immunosuppressive and carcinogenic properties.
- The study aimed to explore the effectiveness of pig lymphocytes in the MTT cytotoxicity test as a more accessible alternative to human lymphocytes and assess the toxic effects of the mycotoxins both individually and in combination.
- Results indicated that exposure to these toxins led to decreased lymphocyte viability over time, with FB1 being less toxic than OTA, and a synergistic effect was observed when both toxins were combined, particularly affecting pig lymphocytes more severely.