Publications by authors named "Michael D Brasino"
In this work, isothermal rolling circle amplification (RCA) of the multi-kilobase genome of engineered filamentous bacteriophage is used to report the presence and identification of specific protein analytes in solution. First, bacteriophages were chosen as sensing platforms because peptides or antibodies that bind medically relevant targets can be isolated through phage display or expressed as fusions to their p3 and p8 coat proteins. Second, the circular, single-stranded genome contained within the phage serves as a natural large DNA template for a RCA reaction to rapidly generate exponential amounts of double stranded DNA in a single isothermal step that can be easily detected using low-cost fluorescent nucleic acid stains.
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- Precise integration of materials at the nanoscale is crucial for developing advanced electronic and photonic devices.
- Researchers employed an atomic force microscope (AFM) to create carbon-free silicon (Si), germanium (Ge), and silicon-germanium (SiGe) nanostructures with exact control over their size and shape.
- The method can be scaled up for larger areas using specialized conducting stamps, and it can be adapted to work with various semiconductor materials by choosing different chemical precursors.