Asparagusic Golgi Trackers.

Thiol-mediated uptake (TMU) is thought to occur through dynamic covalent cascade exchange networks. Here we show that the cascade accounting for TMU of asparagusic acid derivatives (AspA) ends in the Golgi apparatus (G) and shifts from disulfide to thioester exchange with palmitoyl transferases as the final exchange partner. As a result, AspA combined with pH-sensitive fluoresceins, red-shifted silicon-rhodamines, or mechanosensitive flipper probes selectively labels the Golgi apparatus in fluorescence microscopy images in living and fixed cells.

Thiol-Mediated Uptake (TMU, TIMEUP).

This account briefly summarizes objectives and progress made so far with the Swiss-ERC AdG entitled Translational Dynamic Covalent Exchange Cascades (TIMEUP).