Revised UV extinction coefficients for nucleoside-5'-monophosphates and unpaired DNA and RNA.

Ultraviolet absorption provides the nearly universal basis for determining concentrations of nucleic acids. Values for the UV extinction coefficients of DNA and RNA rely on the mononucleotide values determined 30-50 years ago. We show that nearly all of the previously published extinction coefficients for the nucleoside-5'-monophosphates are too large, and in error by as much as 7%.

Investigations of the Copper Bromide-2,2'-Dipyridyl System: Hydrothermal Synthesis and Structural Characterization of Molecular Cu(3)Br(4)(C(10)H(8)N(2))(2), One-Dimensional CuBr(2)(C(10)H(8)N(2)), and Two-Dimensional [Cu(2)(OH)(2)(C(10)H(8)N(2))(2)][Cu(4)Br(6)].

The hydrothermal reactions of CuBr(2), CuBr, and 2,2'-dipyridyl have resulted in the synthesis of three new compounds. Cu(3)Br(4)(C(10)H(8)N(2))(2) (1) is a molecular phase; CuBr(2)(C(10)H(8)N(2)) (2) is a one-dimensional chain structure; and [Cu(2)(OH)(2)(C(10)H(8)N(2))(2)][Cu(4)Br(6)] (3) crystallizes in a two-dimensional sheet structure. All three compounds were characterized by single-crystal X-ray diffraction.