Carbohydrate-labeled fluorescent microparticles and their binding to lectins.

The preparation of micrometer-sized, cross-linked poly(p-phenyleneethynylene) (PPE) beads to which simple monosaccharides are attached is reported. Mannose, glucose, and galactose derivatives have been synthesized. The fluorescence properties, size distribution, and morphology of these microparticles have been elucidated through fluorimetry, fluorescence confocal microscopy, and scanning electron microscopy.

Glycoxidized low-density lipoprotein regulates the expression of scavenger receptors in THP-1 macrophages.

Low-density lipoprotein (LDL) in patients with diabetes is subject to modification by both oxidation and glycation. In contrast to oxidized LDL, the biological effects of glycoxidized LDL have not been well characterised. In this study, the effects of oxidized, glycated, glycoxidized and oxidized LDL on scavenger receptor gene expressions, and the induction of oxidized LDL uptake and cholesteryl ester accumulation in THP-1 macrophages were compared.