Publications by authors named "Michael C Chung"
Article Synopsis
- Cancer immunotherapy faces challenges due to poor antigen recognition and an unfriendly tumor microenvironment (TME).
- Researchers developed "onion-like" multi-lamellar RNA lipid particle aggregates (LPAs) that enhance the delivery and effectiveness of tumor mRNA antigens by activating specific immune responses in the body.
- In studies with dogs and humans, RNA-LPAs showed promising results, improving survival rates and triggering strong immune reactions against tumors, suggesting they could be a breakthrough method for cancer treatment.
Article Synopsis
- Protein clustering is vital for cell functions and related to diseases, but traditional methods struggle to detect small protein oligomers effectively.
- The CluMPS (clusters magnified by phase separation) reporter enhances the visibility of these small clusters, allowing for sensitive detection and quantification of protein aggregates that are otherwise hard to see.
- The study demonstrates CluMPS's capability to identify both pathological and normal protein clusters in cells, supporting its use for advanced research in understanding protein assembly in their natural environment.
Biomechanical forces are of fundamental importance in biology, diseases, and medicine. Mechanobiology is an emerging interdisciplinary field that studies how biological mechanisms are regulated by biomechanical forces and how physical principles can be leveraged to innovate new therapeutic strategies. This article reviews state-of-the-art mechanobiology knowledge about the yes-associated protein (YAP), a key mechanosensitive protein, and its roles in the development of drug resistance in human cancer.
View Article and Find Full Text PDFMessenger RNA (mRNA) has emerged as a remarkable tool for COVID-19 prevention but its use for induction of therapeutic cancer immunotherapy remains limited by poor antigenicity and a regulatory tumor microenvironment (TME). Herein, we develop a facile approach for substantially enhancing immunogenicity of tumor-derived mRNA in lipid-particle (LP) delivery systems. By using mRNA as a molecular bridge with ultrapure liposomes and foregoing helper lipids, we promote the formation of 'onion-like' multi-lamellar RNA-LP aggregates (LPA).
View Article and Find Full Text PDFBackground: Implementing a laboratory-developed test sometimes requires incorporating an unconventional device into the laboratory information system (LIS) and customizing an interface to reduce transcription error and improve turnaround time. Such a custom interface is a necessity for complicated high-volume tests such as 25-OH Vitamin D by liquid chromatography-tandem mass spectrometry (LC-MS/MS) when there is no vendor-or LIS-supplied interface available. Here, we describe our work and experience interfacing a API 5000 LC-MS/MS instrument with our newly implemented LIS, Epic Beaker, using a combination of in-house scripting software and a middleware vendor, Data Innovations.
View Article and Find Full Text PDFBackground: Automated calculations by laboratory information system (LIS) are efficient and accurate ways of providing calculated laboratory test results. Due to the lack of established advanced mathematical functions and equation logic in LIS software, calculations beyond simple arithmetic functions require a tedious workaround. Free and bioavailable testosterone (BT) calculations require a quadratic solver currently unavailable as ready to use the function on most commercial LIS platforms.
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