Demonstration of the efficacy of a Enteritidis live vaccine for chickens according to the current European Pharmacopoeia Monograph.

Since 2013 the efficacy of new live Enteritidis (SE) vaccines for chickens needs to be demonstrated according to European Pharmacopoeia Monograph 04/2013:2520 to receive approval in the EU. The purpose of this study was to determine whether a vaccine licensed since 1999 could also fulfil the required tests of the current guideline. For this, -free chickens (n = 50) were vaccinated on their 2, 46 and 84 day of life with the live attenuated  Enteritidis strain IDT No.

Suitability of n-alkanes and chromium (III) oxide as digestibility markers in calves at the end of the milk feeding period supplemented with a prebiotic.

Prebiotics reveal positive effects on the growth performance of pigs and poultry, and might influence intestinal microflora. This, in consequence, could alter recovery rates of digestibility markers. In the current study, we evaluated the suitability of chromium (III) oxide (CrO) and the synthetic alkanes n-dotriacontane (C32) and n-hexatriacontane (C36) as external markers for digestibility estimation compared with the standard total collection method in calves supplemented with galacto-oligosaccharides.

Lucerne silage in high yielding dairy cows: effect of parity and analysis of reasons in mixed-parity group housing using random regression test-day models.

A study with high-yielding dairy cows was re-analysed in order to test the suitability of lucerne silage separately for primi- and multiparous cows as an alternative to grass silage in maize-based total mixed rations (TMR). Lactation curves were fitted using random regression test-day models for energy corrected milk (ECM) and dry matter intake (DMI) as well as for number and duration of feeder visits (NFV and DFV, respectively). Existing models for ECM and DMI were extended by animal-specific random effects, which were formulated in their dependency on days in milk.

Effect of exogenous fibrolytic enzymes on ruminal fermentation and nutrient digestion in dairy cows.

The aim of the present study was to examine the effects of an exogenous fibrolytic enzyme product applied to a total mixed ration (TMR) prior to feeding on ruminal fermentation, microbial protein synthesis, nutrient digestion, and milk yield and composition. Six multiparous lactating Holstein cows (598 +/- 29 kg initial live weight and 98 +/- 30 days in milk) fitted with rumen and duodenal cannulae were allocated to two treatments in a crossover design over three consecutive 28-d periods. The TMR containing 50% concentrates, 30% corn silage and 20% grass silage on dry matter (DM) basis, was mixed once daily and fed twice a day.

Development of equations for predicting metabolisable energy concentrations in compound feeds for pigs.

In 2006 in Germany new recommendations for the supply of energy and nutrients to pigs were published, including a modification of the energy evaluation on the basis of metabolisable energy for pigs (MES). It was the objective of this meta-analysis to calculate equations that can be used for predicting MES in compound feeds for pigs on the basis of their nutrient concentrations. Data from digestibility studies with a total of 290 compound feeds were provided by different research institutions.