Impact of a Center of Excellence in Confirming or Excluding a Diagnosis of Hypertrophic Cardiomyopathy.

Tertiary hospitals with expertise in hypertrophic cardiomyopathy (HCM) are assuming a greater role in confirming and correcting HCM diagnoses at referring centers. The objectives were to establish the frequency of alternate diagnoses from referring centers and identify predictors of accuracy of an HCM diagnosis from the referring centers. Imaging findings from echocardiography, cardiac computed tomography, and cardiac magnetic resonance imaging (CMR) in 210 patients referred to an HCM Center of Excellence between September 2020 and October 2022 were reviewed.

Noninvasive Imaging for the Asymptomatic Patient: How to Use Imaging to Guide Treatment Goals?

Imaging subclinical atherosclerosis identifies individuals at higher risk of cardiovascular disease through direct visualization before events occur so that preventative measures can be taken. Coronary artery calcium (CAC) scans are the most widely used and studied to identify subclinical atherosclerosis and are most useful in men older than 40 years and women older than 50 years. Coronary computed tomography angiography has high prognostic value and might be the best modality for assessing subclinical atherosclerosis with incremental increase in predictive value over CAC.

4-Methylcyclohexane methanol (MCHM) affects viability, development, and movement of embryos.

Following chemical spill disasters, it is important to estimate the effects of spilled chemicals on humans and the environment. Here we analyzed the toxicological effects of the coal cleaning chemical, 4-methylcyclohexane methanol (MCHM), which was spilled into the Elk River water supply in 2014. The viability of HEK293 T human cell line cultures and embryos was negatively affected, and the addition of the antioxidants alleviated toxicity with MCHM exposure.

Oxidative Stress Responses and Nutrient Starvation in MCHM Treated .

In 2014, the coal cleaning chemical 4-methylcyclohexane methanol (MCHM) spilled into the water supply for 300,000 West Virginians. Initial toxicology tests showed relatively mild results, but the underlying effects on cellular biology were underexplored. Treated wildtype yeast cells grew poorly, but there was only a small decrease in cell viability.

The Polymorphic PolyQ Tail Protein of the Mediator Complex, Med15, Regulates the Variable Response to Diverse Stresses.

The Mediator is composed of multiple subunits conserved from yeast to humans and plays a central role in transcription. The tail components are not required for basal transcription but are required for responses to different stresses. While some stresses are familiar, such as heat, desiccation, and starvation, others are exotic, yet yeast can elicit a successful stress response.

MCHM Acts as a Hydrotrope, Altering the Balance of Metals in Yeast.

While drugs and other industrial chemicals are routinely studied to assess risks, many widely used chemicals have not been thoroughly evaluated. One such chemical, 4-methylcyclohexane methanol (MCHM), is an industrial coal-cleaning chemical that contaminated the drinking water supply in Charleston, WV, USA in 2014. While a wide range of ailments was reported following the spill, little is known about the molecular effects of MCHM exposure.

Transcriptional Profiling of Reveals the Impact of Variation of a Single Transcription Factor on Differential Gene Expression in 4NQO, Fermentable, and Nonfermentable Carbon Sources.

Cellular metabolism can change the potency of a chemical's tumorigenicity. 4-nitroquinoline-1-oxide (4NQO) is a tumorigenic drug widely used on animal models for cancer research. Polymorphisms of the transcription factor Yrr1 confer different levels of resistance to 4NQO in To study how different Yrr1 alleles regulate gene expression leading to resistance, transcriptomes of three isogenic strains carrying different Yrr1 alleles were profiled via RNA sequencing (RNA-Seq) and chromatin immunoprecipitation coupled with sequencing (ChIP-Seq) in the presence and absence of 4NQO.

Primary posterior cruciate-retaining total knee arthroplasty: a comparison of American and Japanese cohorts.

Excessive polyethylene wear is recognized as one of the most important factors affecting the durability of total knee arthroplasty; however, bearing surface wear is a multifactorial problem. The purpose of this study was to identify factors for polyethylene wear and failure in primary posterior cruciate-retaining (PCR) total knee arthroplasty (TKA) in two disparate cohorts (American and Japanese). Seventy-three total knee arthroplasty operations were performed on 48 Japanese patients, and 76 on 63 American patients with noninflammatory arthritides.

A reexamination of stimulus-frequency effects in recognition: two mirrors for low- and high-frequency pseudowords.

The word-frequency mirror effect (more hits and fewer false alarms for low-frequency than for high-frequency words) has intrigued memory researchers, and multiple accounts have been offered to explain the result. In this study, participants were differentially familiarized to various pseudowords in a familiarization phase that spanned multiple weeks. Recognition tests given during the first week of familiarization replicated a result of W.