Codeine dysregulates ribosome biogenesis in with DNA double-strand breaks to chart path to new classes of antibiotics.

Aim: A bacterial genetics-guided approach was utilized for the discovery of new compounds affecting bacterial genome stability.

Materials & Methods: Fungal extracts and fractions were tested for genome instability-mediated antibacterial activity. Interaction assays and RT-qPCR were used to identify compounds that boost the activity of sub-minimum inhibitory concentration streptomycin and obtain insights on the molecular mechanisms of the primary hit compound, respectively.