Publications by authors named "Michael A VanInsberghe"
Article Synopsis
- Long-term issues in chromatin assembly during DNA replication can significantly impact how cells maintain their epigenetic information and decide their fate.
- Researchers studied the effects of quickly removing a key protein called CAF-1, which is crucial for building chromatin, by using advanced techniques like single-cell genomics and live microscopy.
- The loss of CAF-1 slows DNA replication, makes new DNA more accessible, triggers a unique cellular response that reduces histone mRNA levels, and ultimately leads to cell-cycle arrest influenced by p53, highlighting the immediate consequences of faulty chromatin assembly.
Long-term perturbation of chromatin assembly during DNA replication has profound effects on epigenome maintenance and cell fate. The early mechanistic origin of these defects is unknown. Here, we combine acute degradation of Chromatin Assembly Factor 1 (CAF-1), a key player in chromatin assembly, with single-cell genomics, quantitative proteomics, and live-microscopy to uncover these initiating mechanisms in human cells.
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