Differential behavioral engagement of inhibitory interneuron subtypes in the zebra finch brain.

Inhibitory interneurons are highly heterogeneous circuit elements often characterized by cell biological properties, but how these factors relate to specific roles underlying complex behavior remains poorly understood. Using chronic silicon probe recordings, we demonstrate that distinct interneuron groups perform different inhibitory roles within HVC, a song production circuit in the zebra finch forebrain. To link these functional subtypes to molecular identity, we performed two-photon targeted electrophysiological recordings of HVC interneurons followed by post hoc immunohistochemistry of subtype-specific markers.

Stimulation of caudal inferior and middle frontal gyri disrupts planning during spoken interaction.

Turn-taking is a central feature of conversation across languages and cultures. This key social behavior requires numerous sensorimotor and cognitive operations that can be organized into three general phases: comprehension of a partner's turn, preparation of a speaker's own turn, and execution of that turn. Using intracranial electrocorticography, we recently demonstrated that neural activity related to these phases is functionally distinct during turn-taking.

Temporal scaling of motor cortical dynamics reveals hierarchical control of vocal production.

Neocortical activity is thought to mediate voluntary control over vocal production, but the underlying neural mechanisms remain unclear. In a highly vocal rodent, the male Alston's singing mouse, we investigate neural dynamics in the orofacial motor cortex (OMC), a structure critical for vocal behavior. We first describe neural activity that is modulated by component notes (~100 ms), probably representing sensory feedback.

Uncoordinated sleep replay across hemispheres in the zebra finch.

Bilaterally organized brain regions are often simultaneously active in both humans and animal models, but the extent to which the temporal progression of internally generated dynamics is coordinated across hemispheres and how this coordination changes with brain state remain poorly understood. To address these issues, we investigated the zebra finch courtship song (duration: 0.5-1.

De novo assembly and annotation of the singing mouse genome.

Background: Developing genomic resources for a diverse range of species is an important step towards understanding the mechanisms underlying complex traits. Specifically, organisms that exhibit unique and accessible phenotypes-of-interest allow researchers to address questions that may be ill-suited to traditional model organisms. We sequenced the genome and transcriptome of Alston's singing mouse (Scotinomys teguina), an emerging model for social cognition and vocal communication.

A frontal cortical network is critical for language planning during spoken interaction.

Many brain areas exhibit activity correlated with language planning, but the impact of these dynamics on spoken interaction remains unclear. Here we use direct electrical stimulation to transiently perturb cortical function in neurosurgical patient-volunteers performing a question-answer task. Stimulating structures involved in speech motor function evoked diverse articulatory deficits, while perturbations of caudal inferior and middle frontal gyri - which exhibit preparatory activity during conversational turn-taking - led to response errors.

Thalamus drives vocal onsets in the zebra finch courtship song.

While motor cortical circuits contain information related to specific movement parameters, long-range inputs also have a critical role in action execution. Thalamic projections can shape premotor activity and have been suggested to mediate the selection of short, stereotyped actions comprising more complex behaviours. However, the mechanisms by which thalamus interacts with motor cortical circuits to execute such movement sequences remain unknown.

EVALUATION OF EXISTING PUBLIC DOSE LIMITS APPLIED TO RECREATIONAL SPACEFLIGHT.

Establishing realistic radiation dose limits with a solid scientific basis is a key component of the 'as low as reasonably achievable' (ALARA) principle. Although existing occupational dose limits have been established for civil astronauts, with the rise in popularity and technological maturation of the 'space tourism' sector, there does not appear to be considerable discussion on the subject of non-occupational astronaut dose limits. The necessity to come to a collective decision on dose limits and radiation safety procedures for recreational spaceflight is urgent and imperative to maintain ALARA goals, as existing federal dose limits to the public cannot be adequately or universally applied to the space tourism sector.

Neural dynamics in the rodent motor cortex enables flexible control of vocal timing.

Neocortical activity is thought to mediate voluntary control over vocal production, but the underlying neural mechanisms remain unclear. In a highly vocal rodent, the Alston's singing mouse, we investigate neural dynamics in the orofacial motor cortex (OMC), a structure critical for vocal behavior. We first describe neural activity that is modulated by component notes (approx.

A Patient with Concurrent Legionella and COVID-19 Infection in a UK District General Hospital.

A 65 year-old gentleman had been brought to our Respiratory Emergency Department for patients with respiratory symptoms and a possible COVID-19 infection with a 3-day history of shortness of breath (SOB), fever, a productive cough of yellow sputum, and right-sided chest pain. The patient had received both vaccinations at the time and initially reported no travel history, although later it was revealed that he had recently stayed at a hotel. He tested positive for COVID-19 and had hyponatremia and a raised procalcitonin, indicating a bacterial infection as well.

Interventions Risk Evaluation and Management in Aseptic Manufacturing.

Interventions performed by personnel during an aseptic process can be a key source of microbiological contamination of sterile biopharmaceutical products, irrespective of the type of manufacturing system used. Understanding the relative risk of this source of contamination provides valuable information to help make decisions for the design, qualification, validation, operation, monitoring, and evaluation of the aseptic process. These decisions can be used to improve the aseptic process and provide assurance of the sterility of the products.

A Theoretical Framework for Human and Nonhuman Vocal Interaction.

Vocal communication is a critical feature of social interaction across species; however, the relation between such behavior in humans and nonhumans remains unclear. To enable comparative investigation of this topic, we review the literature pertinent to interactive language use and identify the superset of cognitive operations involved in generating communicative action. We posit these functions comprise three intersecting multistep pathways: () the Content Pathway, which selects the movements constituting a response; () the Timing Pathway, which temporally structures responses; and () the Affect Pathway, which modulates response parameters according to internal state.

A speech planning network for interactive language use.

During conversation, people take turns speaking by rapidly responding to their partners while simultaneously avoiding interruption. Such interactions display a remarkable degree of coordination, as gaps between turns are typically about 200 milliseconds-approximately the duration of an eyeblink. These latencies are considerably shorter than those observed in simple word-production tasks, which indicates that speakers often plan their responses while listening to their partners.

Sleep replay reveals premotor circuit structure for a skilled behavior.

Neural circuits often exhibit sequences of activity, but the contribution of local networks to their generation remains unclear. In the zebra finch, song-related premotor sequences within HVC may result from some combination of local connectivity and long-range thalamic inputs from nucleus uvaeformis (Uva). Because lesions to either structure abolish song, we examine "sleep replay" using high-density recording methods to reconstruct precise song-related events.

Using focal cooling to link neural dynamics and behavior.

Establishing a causal link between neural function and behavioral output has remained a challenging problem. Commonly used perturbation techniques enable unprecedented control over intrinsic activity patterns and can effectively identify crucial circuit elements important for specific behaviors. However, these approaches may severely disrupt activity, precluding an investigation into the behavioral relevance of moment-to-moment neural dynamics within a specified brain region.

Mental Well-Being in UK Higher Education During Covid-19: Do Students Trust Universities and the Government?

This paper draws upon the concept of recreancy to examine the mental well-being of university students during the Covid-19 pandemic. Briefly, recreancy is loss of societal trust that results when institutional actors can no longer be counted on to perform their responsibilities. Our study of mental well-being and recreancy focuses on the role of universities and government regulators within the education sector.

Examining the relationship between child holiday club attendance and parental mental wellbeing.

Objectives: This paper reports results of an evaluation of 17 holiday clubs located throughout North East England that ran during the summer of 2017, designed to reduced summertime food insecurity.

Study Design: Questionnaire administed to parents/caregivers of children who attended a holiday club.

Methods: Ordinary Least Squares regression models were used to predict Warwick-Edinburg Mental Wellbeing scale scores measuring parental mental wellbeing.

Pediatric Index of Mortality 3-An Evaluation of Function Among ICUs In South Africa.

Objectives: To evaluate the performance of the Pediatric Index of Mortality 3 as mortality risk assessment model.

Design: This prospective study included all admissions 30 days to 18 years old for 12 months during 2016 and 2017. Data gathered included the following: age and gender, diagnosis and reason for PICU admission, data specific for the Pediatric Index of Mortality 3 calculation, PICU outcomes (death or survival), and length of PICU stay.

Psychosocial impacts of post-disaster compensation processes: Community-wide avoidance behaviors.

Rationale: There is a small but growing body of literature on litigation- and compensation-related stress after disasters. Results of these studies are consistent and unsurprising: compensation processes are a source of stress to plaintiffs and their families. "Litigation Response Syndrome"-anxiety, stress, and depression-is common among those exposed to the pressures of litigation (Lees-Haley 1988).

Focal Cortical Surface Cooling is a Novel and Safe Method for Intraoperative Functional Brain Mapping.

Objective: Electric cortical stimulation (ECS) has been the gold standard for intraoperative functional mapping in neurosurgery, yet it carries the risk of induced seizures. We assess the safety of focal cortical cooling (CC) as a potential alternative to ECS.

Methods: We reviewed 40 patients (13 with tumor and 27 with mesial temporal lobe epilepsy) who underwent intraoperative CC at the University of Iowa Hospital and Clinics (CC group), of whom 38 underwent ECS preceding CC.

The Climate Change-Temperature-Crime Hypothesis: Evidence from a Sample of 15 Large US Cities, 2002 to 2015.

Drawing on prior studies, green criminologists have hypothesized that climate change will both raise the mean temperature and the level of crime. We call this the "climate change-temperature-crime hypothesis" ("CC-T-C"). This hypothesis is an extension of research performed on temperature and crime at the individual level.

Local Axonal Conduction Shapes the Spatiotemporal Properties of Neural Sequences.

Sequential activation of neurons has been observed during various behavioral and cognitive processes, but the underlying circuit mechanisms remain poorly understood. Here, we investigate premotor sequences in HVC (proper name) of the adult zebra finch forebrain that are central to the performance of the temporally precise courtship song. We use high-density silicon probes to measure song-related population activity, and we compare these observations with predictions from a range of network models.

Shale Gas Development and Community Distress: Evidence from England.

This research examines psychosocial stress associated with shale gas development through the narratives of residents and the Revised Impact of Event Scale (IES-R). We carried out our research in three of England's communities impacted by shale gas development. To gather data, we conducted qualitative interviews and engaged in participant observation in all three communities and conducted a quantitative survey of residents.

Singing mice.

A Quick guide to singing mice.