Real-time monitoring of the reaction between aniline and acetonylacetone using extractive electorspray ionization tandem mass spectrometry.

In this work an on-line monitoring method was developed to study the mechanism of acetic acid catalyzed reaction between aniline and acetonylacetone using extractive electorspray ionization-tandem mass spectrometry (EESI-MS). The signals of reactants, intermediates and various byproducts were continuously detected as a function of reaction time. The chemical assignment of each signal was done via multi-stage collision induced dissociation (CID) analysis, and the reaction mechanism between aniline and acetonylacetone was deduced based on the generated molecular ions and fragment ions.

Cardioprotective effects of total flavonoids from Jinhe Yangxin prescription by activating the PI3K/Akt signaling pathway in myocardial ischemia injury.

The purpose of the present study was to investigate the cardioprotective effects of total flavonoids of Jinhe yangxin prescription (JHTF) on myocardial ischemia (MI) injury rats induced by Isoproterenol (ISO) and explore the potential mechanisms underlying these effects. 128 male rats were randomized into 8 groups: Control, Model, Positive, JHTF-H (2.64 g/kg/d), JHTF-M (1.

[Protective Effect of Component Compatibility Sini Decoction on Hypothyroidism Induced Renal Damage in Rats].

Objective: To investigate the protective effect of component compatibility Sini Decoction on hypothyroidism induced renal damage in rats.

Methods: The hypothyroidism model were established by 0.1% propylthiouracil (PTU) solution 10 m/kg for consecutive 15 days.

[Studies on chemical structure of complexes compounds formed by acid and alkaline components of pair medicines in decoction].

Objective: To study the chemical structures of macromolecular complexes formed by aconitine and glycyrrhizic acid, aconitine and rhein, berberine and rhein, ephedrine and glycyrrhizic acid in decoctions.

Method: The binding energy of reactants and products was determined by X-ray spectrography, the ground level charge distribution of products was caculated by density function theory of quantum chemistry.

Result: The binding energy of aconitine and glycyrrhizic acid, aconitine and rhein, berberine and rhein were changed after reaction.

[Compatability chemistry of acid-alkaline pair medicine of Ephedra sinica and Glycyrrhiza uralencis in Maxing Shigan decoction].

Objective: To investigate the change regularity of ephedrine and glyrihhzine acid in Ephedra sinila and Glycyrrhiza uralencis pair medicines and in Maxing Shigan decoction.

Method: The contents of ephedrine and glycyrrhizic acid were determined by HPLC in samples of E. sinica extracts, G.

[Compatibility chemistry of acid-alkaline pair medicine of Dahuang and Huangbai in Dahuang Xiaoshi decoction].

Objective: To determine the change pattern of alkaline and acid components of Dahuang and Huangbai amoung different combinations in Dahuang Xiaoshi decotion.

Method: The contents of anthraquinones and berberine were determined by HPLC in samples of Dahuang extracts, Huangbai extracts, Dahuang and Huangbai pair medicine extracts, and extracts of whole recipe.

Result: The contents of anthraquinones and berberine were decreased with the changes of combinations: from component medicinal material alone to pair medicines, and to whole recipe.

[Compatability chemistry of acid-alkaline pair medicine of dahuang and Fuzi in Dahuang Fuzi decoction].

Objective: To determine the change pattern of alkaline and acid components of Dahuang and Fuzi in different combinations.

Method: The contents of anthraquinones and aconite alkaloids were determined by HPLC in samples of Dahuang extracts, Fuzi extracts, Dahuang and Fuzi pair medicines extracts, and extracts of whole recipe.

Result: As for Dahuang, the contents of anthraquinones were decreased with changes of combination: higher contents were in decoctions of Dahuang alone, lower contents were in decoctions of pair medicines, and the lowest contents were in decoctions of whole recipe.