UV-filter benzophenones suppress human, pig, rat, and mouse 11β-hydroxysteroid dehydrogenase 1: Structure-activity relationship and in silico docking analysis.

Benzophenone chemicals (BPs) have been developed to prevent the adverse effects of UV radiation and they are widely contaminated. 11β-Hydroxysteroid dehydrogenase 1 (11β-HSD1) catalyze the conversion of inactive glucocorticoid to active glucocorticoid, playing critical role in many physiological function. However, the direct effect of BPs on human, pig, rat, and mouse 11β-HSD1 remains unclear.

Demethoxylation of curcumin enhances its inhibition on human and rat 17β-hydroxysteroid dehydrogenase 3: QSAR structure-activity relationship and in silico docking analysis.

Curcuminoids have many pharmacological effects. They or their metabolites may have side effects by suppressing 17β-hydroxysteroid dehydrogenase 3 (17β-HSD3). Herein, we investigated the inhibition of curcuminoids and their metabolites on human and rat 17β-HSD3 and analyzed their structure-activity relationship (SAR) and performed in silico docking.

Bisphenol a alternatives suppress human and rat aromatase activity: QSAR structure-activity relationship and in silico docking analysis.

The use of alternative substances to replace bisphenol A (BPA) has been encouraged. The objective of this study was to evaluate the effects of BPA and 9 BPA alternatives on human and rat aromatase (CYP19A1) in human and rat placental microsomes. The results revealed that bisphenol A, AP, B, C, E, F, FL, S, and Z, and 4,4'-thiodiphenol (TDP) inhibited human CYP19A1 and bisphenol A, AP, B, C, FL, Z, and TDP inhibited rat CYP19A1.

Azole fungicides inhibit human and rat gonadal 3β-hydroxysteroid dehydrogenases: Structure-activity relationship and in silico docking analysis.

Azole fungicides are widely used in the agricultural industry to control fungal infections in crops. However, recent studies have shown that some azole fungicides inhibit the activity of 3β-hydroxysteroid dehydrogenases (3β-HSDs) in the gonads. Out of the 16 azole fungicides tested, 8 were found to inhibit human KGN cell 3β-HSD2 with IC values of less than 100 μM.

[Upgrading microbial strains for fermentation industry].

Fermentation is a green, low-carbon and sustainable process for the production of food, chemicals, fuels, and materials by using microbial strains as biocatalysts and renewable resources such as starch and biomass as feedstocks. China has the world's largest fermentation industry, the scale of amino acids, vitamins, and some other fermentation products accounted for 60%-80% of the global market share. The development of fermentation industry is of great significance for the strategic goal of "carbon neutralization and carbon peak" and the development of bioeconomy.

Characterization of a Riboflavin-Producing Mutant of Isolated by Droplet-Based Microfluidics Screening.

is one of the commonly used industrial strains for riboflavin production. High-throughput screening is useful in biotechnology, but there are still an insufficient number of articles focusing on improving the riboflavin production of by this powerful tool. With droplet-based microfluidics technology, single cells can be encapsulated in droplets.

Integrated Urinalysis Devices Based on Interface-Engineered Field-Effect Transistor Biosensors Incorporated With Electronic Circuits.

Urinalysis is attractive in non-invasive early diagnosis of bladder cancer compared with clinical gold standard cystoscopy. However, the trace bladder tumor biomarkers in urine and the particularly complex urine environment pose significant challenges for urinalysis. Here, a clinically adoptable urinalysis device that integrates molecular-specificity indium gallium zinc oxide field-effect transistor (IGZO FET) biosensor arrays, a device control panel, and an internet terminal for directly analyzing five bladder-tumor-associated proteins in clinical urine samples, is reported for bladder cancer diagnosis and classification.

Improving the Production of Riboflavin by Introducing a Mutant Ribulose 5-Phosphate 3-Epimerase Gene in .

Ribulose 5-phosphate (Ru5P) and guanosine 5'-triphosphate (GTP) are two key precursors of riboflavin, whereby Ru5P is also a precursor of GTP. Ribulose 5-phosphate 3-epimerase (Rpe) catalyzes the conversion of ribulose 5-phosphate into xylulose 5-phosphate. Inactivation of Rpe can reduce the consumption of Ru5P, enhancing the carbon flux toward riboflavin biosynthesis.

Study of the Characteristics of Two Immobilized Microbial Materials in Degradation and Evolution of Petroleum Hydrocarbon.

To enhance the degradation efficiency of oily wastewater, polyacrylamide (PAM)-sodium alginate (SA) and poly(vinyl alcohol) (PVA)-sodium alginate (SA) were mixed and used as spherical supporting materials for the immobilization of microbials, which were employed as a platform to study the degradation of total petroleum hydrocarbons (TPHs) in the oily wastewater. The degradation and evolution of normal paraffin (n-paraffin) series have been studied by determining the crude oil group composition of the residual oils by the gas chromatography-mass spectrometry (GC-MS) analysis. The results show that the half-lives of the PAM-SA-immobilized microorganisms are 6.

[Expression and significance of Ki-67, PI3K and Beclin1 in oral squamous cell carcinoma].

Purpose: To detect the expression and significance of Ki-67, PI3K and Beclin1 in oral squamous cell carcinoma (OSCC).

Methods: Thirty patients with OSCC admitted to Nanjing Stomatology Hospital from January 2017 to December 2018 were selected. All specimens were harvested and treated with immunohistochemical staining.

Superhydrophilic and Oleophobic Porous Architectures Based on Basalt Fibers as Oil-Repellent Photothermal Materials for Solar Steam Generation.

The construction of efficient solar steam generation systems is of great importance for a range of purposes, such as desalination, distillation, and sterilization. In this study, superhydrophilic and oleophobic porous architectures are prepared by carbonization of a mixture of basalt fibers and tissue paper (BFT) followed by an oleophobic modification (O-BFT) to form oil-repellent photothermal materials for efficient solar steam generation. The as-prepared O-BFT shows high porosity, superior light absorption, and good thermal insulation (thermal conductivity=0.

Video-Based Person Re-Identification by an End-To-End Learning Architecture with Hybrid Deep Appearance-Temporal Feature.

Video-based person re-identification is an important task with the challenges of lighting variation, low-resolution images, background clutter, occlusion, and human appearance similarity in the multi-camera visual sensor networks. In this paper, we propose a video-based person re-identification method called the end-to-end learning architecture with hybrid deep appearance-temporal feature. It can learn the appearance features of pivotal frames, the temporal features, and the independent distance metric of different features.

Engineering a vitamin B high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti.

Background: As a very important coenzyme in the cell metabolism, Vitamin B (cobalamin, VB) has been widely used in food and medicine fields. The complete biosynthesis of VB requires approximately 30 genes, but overexpression of these genes did not result in expected increase of VB production. High-yield VB-producing strains are usually obtained by mutagenesis treatments, thus developing an efficient screening approach is urgently needed.

Nicotinamide Phosphoribosyltransferase Upregulation by Phenylephrine Reduces Radiation Injury in Submandibular Gland.

Purpose: Radiation therapy for head and neck cancer commonly leads to radiation sialadenitis. Emerging evidence has indicated that phenylephrine pretreatment reduces radiosensitivity in the salivary gland; however, the underlying cytoprotective mechanism remains unclear. Nicotinamide phosphoribosyltransferase (NAMPT) is not only a key enzyme for the nicotinamide adenine dinucleotide salvage pathway, but also a cytokine participating in cell survival, metabolism, and longevity, with a broad effect on cellular functions in physiology and pathology.

A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320.

Vitamin B12 (Cobalamin, VB12) has several physiological functions and is widely used in pharmaceutical and food industries. A new unicellular species was extracted from China farmland, and the strain could produce VB12 which was identified by HPLC and HPLC-MS/MS. 16S rDNA analysis reveals this strain belongs to the species Sinorhizobium meliloti and we named it S.

Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis.

Toxin-antitoxin (TA) systems are widely prevalent in the genomes of bacteria and archaea. These modules have been identified in Escherichia coli and various other bacteria. However, their presence in the genome of Streptococcus suis, an important zoonotic pathogen, has received little attention.

Whole-cell method for phenol detection based on the color reaction of phenol with 4-aminoantipyrine catalyzed by CotA laccase on endospore surfaces.

A green method for phenol spectrophotometric determination was developed based on the color reaction of phenol with 4-aminoantipyrine catalyzed by addition of Bacillus amyloliquefaciens endospores in the presence of O2. The catalytic activity of the endospores may be attributed to the presence of coat protein A on the cell surfaces. This deduction was confirmed by cotA gene knock-out from B.