The Combination of and Its Engineered Strain Expressing dsRNA Increases the Toxicity against .

RNA interference (RNAi) has been developed and used as an emerging strategy for pest management. Here, an entomopathogen () was used to express the dsRNA for the control of . A vector containing a 325-bp fragment of the conserved region of arginine kinase gene () flanking in two ends with the promoter Pro3α was developed and transferred into 8010 and BMB171, and consequently engineered strains 8010AKi and BMB171AKi expressing dsRNA of were developed.

Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in .

DsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in are still unknown. We identified the full-length cDNAs of , , , and .

[The value of bispectral index in the unconscious patients with acute brain injury due to different pathogenic factors].

Objective: To observe the differences in bispectral index (BIS) in unconscious patients with acute brain injury due to different pathogenic factors, and approach its clinical significance.

Methods: A retrospective study was conducted. One hundred and twenty-two unconscious patients with acute brain injured admitted to the intensive care unit (ICU) from March 2009 to August 2012 were involved.

[Evaluation of continuous monitoring of bispectral index in prognosis in patients with acute brain injury].

Objective: To discuss the effect of continuous monitoring of bispectral index (BIS) on the prognosis of patients with acute brain injury.

Methods: A retrospective study was carried out, 61 patients with acute brain injury admitted to the intensive care unit (ICU) from March 2009 to July 2010 were divided into survival group (n=25) and death group (n=36). The BIS was continuously monitored for 12 hours within the first 3 days or 24 hours after stoppage of sedative after admission to ICU.

[Role of neuron specific enolase and S100 protein in evaluation of brain damage in patients resuscitated from cardiac arrest].

Objective: To investigate the prognostic value of serum neuron specific enolase (NSE) and S100 protein in evaluation of brain damage in patients resuscitated from cardiac arrest (CA).

Methods: According to whether the patients regained consciousness after 6 months or not, 25 patients after cardiopulmonary resuscitation (CPR) were divided into 2 groups, and blood samples were obtained for determination of NSE and S100 protein at 2, 12, 24, 48 and 72 hours after recovery of spontaneous circulation (ROSC), then the values at each time point were compared between 2 groups and also with that of 7 healthy volunteers. Receiver operator characteristic (ROC) curves of serum NSE and S100 protein were depicted and used area under curve (AUS) to scale the ability in evaluating the state of consciousness in patients after CPR.