Masquelet Inspired in Vivo Engineered Extracellular Matrix as Functional Periosteum for Bone Defect Repair and Reconstruction.

The Masquelet technique that combines a foreign body reaction (FBR)-induced vascularized tissue membrane with staged bone grafting for reconstruction of segmental bone defect has gained wide attention in Orthopedic surgery. The success of Masquelet hinges on its ability to promote formation of a "periosteum-like" FBR-induced membrane at the bone defect site. Inspired by Masquelet's technique, here a novel approach is devised to create periosteum mimetics from decellularized extracellular matrix (dECM), engineered in vivo through FBR, for reconstruction of segmental bone defects.

An Outbreak of Foodborne Botulism Caused by BoNT/A3 in Pickled Eggs - Weihai City, Shandong Province, China, July 2024.

What Is Already Known About This Topic?: Foodborne botulism is caused by botulinum neurotoxin (BoNT). () is a strictly anaerobic, Gram-positive bacterium, which is a key pathogen capable of producing BoNT. BoNTs can be classified into seven serotypes (A to G) based on their antigenic properties.

Uncovering the mechanisms underlying the efficacy of probiotic strains in mitigating food allergies: an emphasis on gut microbiota and indoleacrylic acid.

Food allergies manifest as systemic or digestive allergic responses induced by food allergens, and their progression has been demonstrated to be intimately associated with the host's gut microbiota. Our preceding investigation has revealed that the probiotic strains CCFM1189 and CCFM1190 possess the capability to mitigate the symptoms of food allergy in mice. However, the underlying mechanisms and material foundations through which these probiotic strains exert their effects remain enigmatic.

A novel directed enzymolysis strategy for the preparation of umami peptides in Stropharia rugosoannulata and its mechanism of taste perception.

This study aimed to explore the effect of directed enzymolysis on the umami characteristics of S. rugosoannulata, clarify the flavour formation mechanism of umami peptides. We expressed a new aminopeptidase (DNPEP) and obtained the umami peptides of S.

Microwave replacing traditional concentrating process to promote the synthesis of mumefural in Prunus mume extract.

Mumefural (MF), the primary functional ingredient of prunus mume extract that enhances blood fluidity, is produced by boiling the prunus mume juice for 24-48 h in a traditional one-step concentration process. Low MF yields and long processing time are two disadvantages of this process. This study introduced microwaves into the traditional one-step concentration process of prunus mume extract for increasing MF content.

Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

This Letter reports a search for charge-parity (CP) symmetry violating nonstandard interactions (NSI) of neutrinos with matter using the NOvA Experiment, and examines their effects on the determination of the standard oscillation parameters. Data from ν_{μ}(ν[over ¯]_{μ})→ν_{μ}(ν[over ¯]_{μ}) and ν_{μ}(ν[over ¯]_{μ})→ν_{e}(ν[over ¯]_{e}) oscillation channels are used to measure the effect of the NSI parameters ϵ_{eμ} and ϵ_{eτ}. With 90% CL the magnitudes of the NSI couplings are constrained to be |ϵ_{eμ}|≲0.

Noise-aware dynamic image denoising and positron range correction for Rubidium-82 cardiac PET imaging via self-supervision.

Rubidium-82 (Rb) is a radioactive isotope widely used for cardiac PET imaging. Despite numerous benefits of Rb, there are several factors that limits its image quality and quantitative accuracy. First, the short half-life of Rb results in noisy dynamic frames.

Direct Synthesis of Sulfinylated Phenanthrenes via BF-Promoted Annulation of 2-Alkynyl Biaryls with Arylsulfinic Acids.

A novel approach to sulfinylated phenanthrenes has been developed by BF-promoted annulation of 2-alkynyl biaryls with arylsulfinic acids. This approach utilizes readily available arylsulfinic acids as a sulfinyl source and affords a variety of sulfinylated phenanthrenes in good to excellent yields under simple and mild reaction conditions.

Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.

Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

Bioinspired peptide sensors with tailorable structure for specific and in-situ tracking of Hg biodistribution in living cells upon acute exposure.

Metal-biomolecule interactions that are ubiquitous in nature provide fundamental knowledge and rich structural motifs for the development of functional molecules and smart sensors. In this work, inspired by the active sites in metalloproteins, a biomimetic peptide sensor was designed for the selective recognition and activatable sensing of Hg in living biosystems. Tetraphenylethylene (TPE) with typical aggregation-induced emission (AIE) behavior, was introduced as the activatable signal transducer to enable high signal-to-background signaling.

Controllable Ultrathin Nickel Nanoislands With Dense Discrete Space Charge Regions: Steering Hole Extraction for High-Performance Underwater Multispectral Weak-Light Photodetection.

Undersea optical communication (UOC) is vital for ocean exploration and military applications. In the dim-light underwater environment, photodetectors must maximize photon utilization by minimizing optical losses and carrier recombination. This can be achieved by integrating ultrathin metal nanostructures with photocatalysts to form Schottky junctions, which enhance charge separation and injection while mitigating metal-induced light shading.

A survey of bridging bone on chest radiography shows a greater than expected prevalence of marginal syndesmophytes.

Background: The recognition of thin marginal spinal syndesmophytes is important, in part due to their association with non-traumatic or mildly traumatic vertebral fractures.

Purpose: To determine a lower limit on the prevalence of marginal spinal syndesmophytes using chest radiographs.

Material And Methods: We conducted a retrospective analysis of 500 chest radiographs, assessing the prevalence of thin marginal syndesmophytes, bridging or near-bridging osteophytes, and flowing paravertebral ossifications in the thoracic intervertebral discs among individuals aged 16 years and older in a North American city.

Causal links between circulatory inflammatory cytokines and risk of digestive polyps: a Mendelian randomization analysis.

Background: There is a high morbidity of polyps in the digestive tract, and certain subtypes of polyps are thought to induce cancer progression and often recur, which may be associated with chronic inflammation. Mendelian randomization (MR) can help identify potential causative relationships and inform early treatment action.

Methods: We performed a bidirectional two-sample MR analysis implementing the results from genome-wide association studies for 41 serum cytokines from 8,293 Finnish individuals, and three types of polyps from European ancestry, respectively, including gastric polyp (6,155 cases vs.

Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.

Impaired organelle-specific protein import triggers a variety of cellular stress responses, including adaptive pathways to balance protein homeostasis. Most of the previous studies focus on the cellular stress response triggered by misfolded proteins or defective protein import in the endoplasmic reticulum or mitochondria. However, little is known about the cellular stress response to impaired protein import in the peroxisome, an understudied organelle that has recently emerged as a key signaling hub for cellular and metabolic homeostasis.

Characterization and functional analysis of chicken promyelocytic leukemia protein.

In mammals, promyelocytic leukemia (PML) protein, also named as TRIM19, is the key component of nuclear membrane-less sub structures PML nuclear bodies (PML-NB) or nuclear domains 10 (ND10). PML-NBs are dynamic foci that consist of numerous permanently or transiently associated proteins. The mammalian PMLs are involved in the regulation of various cellular pathways, including apoptosis, intrinsic and innate antiviral immunity, cell cycle, DNA damage, senescence and etc.

Combined DeRitis ratio and alkaline phosphatase on the prediction of portal vein tumor thrombosis in patients with hepatocellular carcinoma.

Portal vein tumor thrombosis (PVTT) is one of the common complications of HCC and represents a sign of poor prognosis. PVTT signifies advanced liver cancer, deteriorating liver function, and heightened susceptibility to intrahepatic dissemination, systemic metastasis, and complications related to portal hypertension. It is important to seek novel strategies for PVTT arising from HCC.

Single-cell transcriptomic analysis of the senescent microenvironment in bone metastasis.

Bone metastasis (BM) is a mortality-related event of late-stage cancer, with non-small cell lung cancer (NSCLC) being a common origin for BM. However, the detailed molecular profiling of the metastatic bone ecosystem is not fully understood, hindering the development of effective therapies for advanced patients. In this study, we examined the cellular heterogeneity between primary tumours and BM from tissues and peripheral blood by single-cell transcriptomic analysis, which was verified using multiplex immunofluorescence staining and public datasets.

Selective oxidation of methane to C products over Au-CeO by photon-phonon co-driven catalysis.

Direct methane conversion to high-value chemicals under mild conditions is attractive yet challenging due to the inertness of methane and the high reactivity of valuable products. This work presents an efficient and selective strategy to achieve direct methane conversion through the oxidative coupling of methane over a visible-responsive Au-loaded CeO by photon-phonon co-driven catalysis. A record-high ethane yield of 755 μmol h (15,100 μmol g h) and selectivity of 93% are achieved under optimised reaction conditions, corresponding to an apparent quantum efficiency of 12% at 365 nm.

Aerobic Training and Ointment Effect on range of motion, von Willebrand factor, vascular endothelial factor and Femoral Artery Hemodynamics in Joint Contracture: A Rat Model.

Objective: Using a rat model, we investigated the effect of multidisciplinary rehabilitation, including aerobic training and ointment, on the ROM, vWF, VEGF content, and femoral artery hemodynamics in rats with joint contracture.

Methods: A total of 44 Wistar rats were divided into the normal control group (NC, eight rats) and the experimental group (EG). A joint contracture model was established for the rats in the EG group by an external fixator.

Towards High-Performance Pockels Effect-Based Modulators: Review and Projections.

The ever-increasing demand for high-speed data transmission in telecommunications and data centers has driven the development of advanced on-chip integrated electro-optic modulators. Silicon modulators, constrained by the relatively weak carrier dispersion effect, face challenges in meeting the stringent requirements of next-generation photonic integrated circuits. Consequently, there has been a growing interest in Pockels effect-based electro-optic modulators, leveraging ferroelectric materials like LiNbO, BaTiO, PZT, and LaTiO.