Integrated analysis of single-cell and bulk RNA sequencing identifies APOC1 as a biomarker and therapeutic target for G0/G1 cell cycle arrest in cholangiocarcinoma.

Cholangiocarcinoma is characterized by its high malignancy, frequent recurrence and insensitivity to conventional radiotherapy and chemotherapy. This resistance may be associated with the presence of cells in the G0/G1 arrest phase within the cancer. Cancer cells in the G0/G1 phase are resistant to therapies targeting actively dividing cells, allowing them to evade conventional adjuvant treatments and survive.

The six whole mitochondrial genomes for the species: features, evolution and phylogeny.

In this study, the complete mitogenomes of three species ( ZM79-3, ZM33-4 and sp. ZM41-5) were sequenced, assembled and compared with the other three previously sequenced mitogenomes ( VNIIKR SE Dcaul3, MSPL 10-6 and VNIIKR SE Dps12). The six mitogenomes were found to be circular DNA molecules, with lengths ranging from 53,646 bp to 108,865 bp.

Hard example mining in Multi-Instance Learning for Whole-Slide Image Classification.

Multiple instance learning(MIL) has shown superior performance in the classification of whole-slide images(WSIs). The implementation of multiple instance learning for WSI classification typically involves two components, i.e.

Innovative design concepts in tumor-targeting peptide-drug conjugates: Insights into emerging applications.

Peptide-drug conjugates (PDCs) have emerged as a promising strategy in cancer therapy, offering improved therapeutic efficacy and reduced toxicity. Compared to antibody-drug conjugates (ADCs) and small molecule-drug conjugates (SMDCs), PDCs possess distinct advantages, such as lower immunogenicity, improved tumor penetration, and simpler synthesis. This review discusses the latest advancements in PDC design, including novel peptide targeting mechanisms, linker selection, and formulation improvements for increased stability.

More Cyberbullying, Less Happiness, and More Injustice-Psychological Changes During the Pericyberbullying Period: Quantitative Study Based on Social Media Data.

Background: The phenomenon of cyberbullying is becoming increasingly severe, and many studies focus on the negative psychological impacts of cyberbullying survivors. However, current survey methods cannot provide direct and reliable evidence of the short-term psychological effects of cyberbullying survivors, as it is impractical to measure psychological changes before and after such an unpredictable event in a short period.

Objective: This study aims to explore the psychological impacts of cyberbullying on survivors during the pericyberbullying period, defined as the critical time frame surrounding the first cyberbullying incident, encompassing the psychological changes before, during, and after the event.

Early Prediction of Death Risk in Progressive Nasopharyngeal Carcinoma Using Radiomics Nomogram Based on Clinical Semantic Multi-Parameter Magnetic Resonance Imaging.

Patients with recurrent or/and metastatic nasopharyngeal carcinoma (NPC) have a notably low survival rate. Our primary objective in this study is to establish a comprehensive nomogram model based on clinical factors, semantic features, and multi-parameter magnetic resonance imaging (MRI) radiomic features, and to predict the risk of mortality in patients with progressive NPC following intensity-modulated radiation therapy. A retrospective study, including 110 patients with recurrent or/and metastatic NPC who underwent treatment at the Zhejiang Cancer Hospital between June 2012 and December 2016, was conducted.

The assessing of clinical relevance of chromosomal microarray analysis in the prenatal diagnosis of fetal growth restriction.

Objective: Chromosomal variations are known to play a role in the etiology of fetal growth restriction (FGR). Here, we intend to investigate the significance of Chromosomal Microarray Analysis (CMA) in the prenatal diagnosis of definite FGR.

Method: 182 pregnant women with FGR participated in our study, undergoing CMA to identify chromosomal abnormalities.

Cartilaginous Endplate Damage May be a Root Pathology Underlying Modic Changes on Lumbar Spine MR Images.

Study Design: Rabbit model study.

Objective: To examine whether cartilaginous endplate (CEP) destruction leads to endplate and vertebral marrow signal changes (Modic changes, MCs) on MR images.

Methods: Sixteen adult rabbits were used in the study and underwent an anterolateral procedure to expose the L2-6 intervertebral discs.

Use of long peripheral catheters in the infusion therapy of lung cancer patients.

Objective: To investigate the applicability, safety, and efficacy of long peripheral catheters in the infusion therapy of lung cancer patients.

Methods: A retrospective analysis was conducted to examine the baseline data, catheter placement procedures, associated complications, as well as the costs of catheter placement and maintenance among patients who underwent long peripheral catheter insertion in our lung oncology unit from June to December 2023.

Results: The average catheterization time was approximately 20 min, achieving a success rate of 71.

Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway.

Jianpi Yiqi Busui Prescription (JYBP), a traditional Chinese medicine formula (TCM), is used in the treatment of myasthenia gravis (MG). However, its mechanisms of action still require further clarification. In this study, an experimental autoimmune MG (EAMG) rat model was established for research.

Global burden of multidrug-resistant tuberculosis in children and adolescents.

Objectives: Multidrug-resistant tuberculosis (MDR-TB) has become a major global public health issue, which has worsened over time owing to changes in disease-related trends. This study aimed to determine global trends in MDR-TB among children and adolescents for strategic health planning.

Methods: A secondary analysis was performed on MDR-TB burden among children and adolescents (<20 years) at the global, regional, and national levels based on sociodemographic index (SDI) quintiles from 1990 to 2019, using the Global Burden of Disease (GBD) 2019 database.

Clinical and Multiorgan Proteomics Characteristics of the Diverse Fatal Phase in Super Elderly Patients With SARS-CoV-2 Infection: A Descriptive Study.

This study aims to identify the risk factors associated with clinical outcomes and the proteomic changes in organs related to fatal SARS-CoV-2 infection within the super-elderly population. This retrospective analysis included all elderly individuals with COVID-19 admitted to the Second Medical Center of PLA General Hospital from December 2022 to January 2023. The follow-up period ended on March 30, 2023.

Multifunctional metal-phenolic nanoparticles with antibacterial and anti-inflammatory effects for osteomyelitis management.

Osteomyelitis is a serious inflammatory disease mostly caused by bacterial infections. It is necessary to simultaneously eradicate bacterial cells and inhibit inflammation in treating osteomyelitis. Herein, we design an innovative zinc ion (Zn)-based nano delivery system for the management of osteomyelitis.

Diagnostic yield of chromosomal microarray to examine the genetic factors associated with fetal aberrant right subclavian artery.

Purpose: The primary objective was to find the risk factors that increase the probability of chromosomal abnormalities in fetal aberrant right subclavian artery (ARSA). Secondary objectives were to assess the factors which impact the adverse pregnancy outcomes in ARSA fetuses.

Methods: This retrospective study included a total of 104 pregnant women whose fetuses were diagnosed with ARSA from January 2020 to July 2024, with subsequent chromosomal microarray analysis (CMA) performed.

Sodium butyrate regulates macrophage polarization by TGR5/β-arrestin2 in vitro.

Background: Macrophages play an important role in the pathogenesis of ulcerative colitis (UC). We will explore the effects of sodium butyrate (SB) on macrophage function.

Methods: The targets of butyric acid were identified using SwissTargetPrediction database and surface plasmon resonance (SPR).

Incidence, risk factors and endoscopic retrieval of proximally migrated pancreatic stents: 25 years of experience in a high-volume center - a case controlled study.

Background And Aim: Proximal migration is one of the complications after pancreatic stenting. This study aims to determine the incidence, risk factors and endoscopic treatment of proximally migrated pancreatic stents.

Methods: A retrospective search of all the endoscopic retrograde cholangiopancreatography (ERCP) records was conducted from 1997 to 2022 in our tertiary center.

LBP-CD155 Liposome Nanovaccine Efficiently Resist Colorectal Cancer and Enhance ICB Therapy.

Background: Colorectal cancer (CRC) is a highly malignant and aggressive gastrointestinal tumor. Due to its weak immunogenicity and limited immune, cell infiltration lead to ineffective clinical outcomes. Therefore, to improve the current prophylaxis and treatment scheme, offering a favorable strategy efficient against CRC is urgently needed.

Nano-Selenium Elevating Leaf Quality and Growth Via Microbial-Regulating Nitrogen Availability Under Ammonium and Nitrate Spraying in Tea Plants.

Nano-selenium fertilizers can promote plant growth and nitrogen availability. However, little information is available on the effects of nano-selenium on tea leaf quality, soil nutrient availability and associated microbe-driven mechanisms. This study examined the effects of nano-selenium on the tea leaf quality and soil nitrogen cycling in 20-year-old tea plantations when the leaves were sprayed with ammonium or nitrate.

Gold Single Atom Doped Defective Nanoporous Copper Octahedrons for Electrocatalytic Reduction of Carbon Dioxide to Ethylene.

Electrocatalytic CO reduction into high-value multicarbon products offers a sustainable approach to closing the anthropogenic carbon cycle and contributing to carbon neutrality, particularly when renewable electricity is used to power the reaction. However, the lack of efficient and durable electrocatalysts with high selectivity for multicarbons severely hinders the practical application of this promising technology. Herein, a nanoporous defective AuCu single-atom alloy (De-AuCu SAA) catalyst is developed through facile low-temperature thermal reduction in hydrogen and a subsequent dealloying process, which shows high selectivity toward ethylene (CH), with a Faradaic efficiency of 52% at the current density of 252 mA cm under a potential of -1.

Electron-phonon coupling and coherent energy superposition induce spin-sensitive orbital degeneracy for enhanced acidic water oxidation.

The development of acid-stable water oxidation electrocatalysts is crucial for high-performance energy conversion devices. Different from traditional nanostructuring, here we employ an innovative microwave-mediated electron-phonon coupling technique to assemble specific Ru atomic patterns (instead of random Ru-particle depositions) on MnCrO surfaces (Ru-MnCrO) in RuCl solution because hydrated Ru-ion complexes can be uniformly activated to replace some Mn sites at nearby Cr-dopants through microwave-triggered energy coherent superposition with molecular rotations and collisions. This selective rearrangement in Ru-MnCrO with particular spin-differentiated polarizations can induce localized spin domain inversion from reversed to parallel direction, which makes Ru-MnCrO demonstrate a high current density of 1.

Efficacy of preoperative pulmonary rehabilitation in lung cancer patients: a systematic review and meta-analysis of randomized controlled trials.

Although previous studies have shown that preoperative pulmonary rehabilitation training may improve postoperative prognosis in patients with lung cancer, the literature included in the existing meta-analysis is highly heterogeneous and lacks effective subgroup analysis. Therefore, an updated meta-analysis is needed to integrate the latest published randomized controlled clinical trials (RCT). This updated analysis was performed to identify the clinical effects of preoperative pulmonary rehabilitation on physical rehabilitation (lung function, activity endurance, and dyspnea), psychological rehabilitation, quality of life, length of hospital stay, and postoperative pulmonary complications in patients with lung cancer.

Leaf nitrogen and phosphorus are more sensitive to environmental factors in dicots than in monocots, globally.

Leaf nitrogen (N) and phosphorus (P) levels provide critical strategies for plant adaptions to changing environments. However, it is unclear whether leaf N and P levels of different plant functional groups (e.g.

Exploring the gut-inflammation connection: A Mendelian randomization study on gut microbiota, inflammatory factors, and uterine fibroids risk.

This study employs Mendelian randomization (MR) approach to investigate the potential causal association between genetic variants associated with gut microbiota, inflammatory factors, and the risk of uterine fibroids development. We extracted data on 211 types of gut microbiota, 91 inflammatory factors, and uterine fibroids occurrence from genome-wide association studies and applied the inverse-variance weighted (IVW) method for analysis. To further assess the robustness of our MR analysis, we conducted sensitivity tests including Cochrane's Q test, the MR-Egger intercept test, the MR-PRESSO global test, and a leave-one-out analysis.

Effect of defects on ballistic transport in a bilayer SnS-based junction with Co intercalated electrodes.

This study theoretically investigates the defect-related electronic structure and transport properties in a device where a semiconductor bilayer SnS (BL-SnS) serves as the central scattering region and bilayer SnS with cobalt atom intercalation (Co-SnS) as the metallic electrodes. The Co-SnS/BL-SnS junction forms an ohmic contact, which is robust to defects. Low contact resistances of 52.

The swelling-induced fractionation strategy to mediate cellulose availability and lignin structural integrity.

We report a facile fractionation strategy using choline hydroxide (ChOH) based alkaline deep eutectic solvents (DES) for whole-component upgrading of bagasse. Through selective lignin and xylan dissolution, along with extensive biomass swelling, high-value lignin-carbohydrate complexes (LCC, with high β-O-4 bond content of 68.9/100 Ar) and high-purity xylan were extracted without compromising cellulose recovery and hydrolysis.