Revealing nutrient limitation status of microorganisms in the soil of a plantation through soil stoichiometry and enzyme metrology.

Exploring nutrient limitation in forest soil holds significant implications for forest tending and management. However, current research on nutrient limitation status of microorganisms in plantations within the Loess Plateau remains insufficient. To investigate soil microbial nutrient limitation of plantations on the Loess Plateau, we selected plantations with different afforestation time series (15, 25, 35, and 45 years) and a pile of barren slope cropland (control) in Yongshou County, Shaanxi Province as the research objects.

Herbaspirillum robiniae sp. nov., isolated from root nodules of Robinia pseudoacacia in a lead-zinc mine.

A novel endophytic bacterium, designated strain HZ10, was isolated from root nodules of Robinia pseudoacacia growing in a lead-zinc mine in Mianxian County, Shaanxi Province, China. The bacterium was Gram-stain-negative, aerobic, motile, slightly curved- and rod-shaped, methyl red-negative, catalase-positive, and did not produce H2S. Strain HZ10 grew at 4-45 °C (optimum, 25-30 °C), pH 5-9 (optimum, pH 7-8) and 0-1 % (w/v) NaCl.

Mitsuaria noduli sp. nov., isolated from the root nodules of Robinia pseudoacacia in a lead-zinc mine.

A novel endophytic bacterium, designated strain HZ7, was isolated from the root nodules of Robinia pseudoacacia growing in a lead-zinc mine in Mianxian County, Shaanxi Province, China. Cells were Gram-reaction-negative, aerobic, motile, rod-shaped, methyl-red-negative, catalase-positive, positive for chitosan-degrading activity and did not produce H2S. Strain HZ7 grew at 4-45 °C (optimum 25-30 °C), at pH 5-9 (optimum pH 7-8) and with 0-1 % (w/v) NaCl.

sp. nov., an actinomycete isolated from the rhizosphere of .

An actinomycete, strain D34, was isolated from a soil sample collected from the rhizosphere of at Yunwu Mountain in Ningxia, north-west China. Strain D34 showed highest 16S rRNA gene sequence similarity to DSM 44229 (99.0 %), NRRL B-16107 (98.

Micromonospora nickelidurans sp. nov., isolated from soil from a nickel-mining site.

An actinomycete, strain K55T, was isolated from a composite soil sample from a nickel mine,collected from Yueyang, Shaanxi Province, PR China. Strain K55T showed 16S rRNA gene sequence similarities of 98.73 %–98.