Integrated microbiomics and metabolomics analysis reveals the influence of gut microbiota on the growth and metabolism of sea cucumber seedlings.

Aims: This study explores the impact of gut microbiota on body metabolites and the growth rate of sea cucumber seedlings.

Methods And Results: A comprehensive analysis using metabolomics and microbiomics was conducted to ascertain the gut microbiota and body metabolites in sea cucumber seedlings exhibiting varying growth rates. Distinct changes in the intestinal flora were observed in correlation with different growth rates of sea cucumber seedlings.

Neiella holothuriorum sp. nov., isolated from the gut of a sea cucumber Apostichopus japonicus.

A Gram-stain negative, aerobic, rod-shaped bacterium, designated 126, was isolated from the intestinal content of a sea cucumber, Apostichopus japonicus, in China. Strain 126 was found to grow optimally at 25-28 °C and pH 7.5-8.

Hypolipidemic effect of soluble dietary fibers prepared from and their effects on the modulation of intestinal microbiota.

The soluble dietary fiber from (ASDF) was successively prepared using enzymolysis combined with spray-drying technology. High-performance liquid chromatography analysis showed that ASDF contained two polysaccharide fractions with the average molecular weight of 2.77 × 10 and 6.

A fucoidan from Sargassum fusiforme with novel structure and its regulatory effects on intestinal microbiota in high-fat diet-fed mice.

A fucoidan SFP, having novel structure, was extracted from Sargassum fusiforme. It had a molecular weight of 703 kDa and was composed of fucose and galactose with the ratio of 73.16:26.

Hot water pretreatment-induced significant metabolite changes in the sea cucumber Apostichopus japonicus.

Hot water pretreatment of sea cucumbers potentially changes nutritional benefits. This study aimed to quantify hot water pretreatment-induced changes in metabolite profiles of sea cucumber body walls. ICP-OES, GC-MS, and LC-MS analyses of untreated- (UT-BW), hot water-treated body walls (HW-BW) of Apostichopus japonicus, and the hot water extract (HW-E) determined significant losses of minerals (25-50% w/w), protein (~11% w/w), carbohydrate (33% w/w), saponins (~41% w/w), and spermidine (100%), a potential antipsychotic from hot water-treated samples.

Structure, enzymatic transformation, anticancer activity of fucoidan and sulphated fucooligosaccharides from Sargassum horneri.

Structure and anticancer activity of fucoidan from Sargassum horneri and from products of its enzymatic transformation were investigated. A gene that encodes fucoidanase ffa1 in the marine bacteria F. algae was identified, cloned and the protein (FFA1) was produced in Escherichia coli.

Three new oleanane-type triterpenoid saponins from the seeds of Celosia cristata L.

Phytochemical investigation of the 1-butanol soluble fraction of 60% ethanol extract of the seeds of Celosia cristata L. led to the identification of three new oleanane-type triterpenoid saponins. Using D and D NMR experiment methods, ESI-MS analysis and acid hydrolysis, their structures were identified as 3-O-[β-D-xylopyranosyl-(1 → 3)-β-D-glucuronopyranosyl]-2β-hydroxy-oleanolic acid-28-O-β-D-glucopyranoside (1), 3-O-[β-D-xylopyranosyl-(1 → 3)-β-D-glucuronopyranosyl]-2β, 23-dihydroxy-oleanolic acid-28-O-β-D-glucopyranoside (2) and 3-O-[β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranosyl]-2-hydroxyl-medicagenic acid-28-O-β-D-glucopyranosyide (3), respectively.

Production of the angiotensin I converting enzyme inhibitory peptides and isolation of four novel peptides from jellyfish (Rhopilema esculentum) protein hydrolysate.

Background: Angiotensin I converting enzyme (ACE) plays an important role in regulating blood pressure in the human body. ACE inhibitory peptides derived from food proteins could exert antihypertensive effects without side effects. Jellyfish (Rhopilema esculentum) is an important fishery resource suitable for production of ACE inhibitory peptides.

Cellulase-assisted extraction, characterization, and bioactivity of polysaccharides from Polygonatum odoratum.

Cellulase-assisted extraction of polysaccharides from Polygonatum odoratum (CPP) was optimized by response surface methodology (RSM) and the extracted CPP's preliminary chemical characteristics, as well as antioxidant and immunomodulatory activities were also investigated. The optimal extraction parameters comprised an extraction temperature of 58.21 °C, an extraction time of 3.

Angiotensin converting enzyme (ACE) inhibitory, antihypertensive and antihyperlipidaemic activities of protein hydrolysates from Rhopilema esculentum.

Angiotensin-converting enzyme (ACE) inhibitory, antihypertensive and antihyperlipidaemic activities of protein hydrolysates (RPH) from the jellyfish Rhopilema esculentum were investigated. R. esculentum was hydrolysed sequentially with pepsin and papain, and then the hydrolysate was ultrafiltered with a 2000 Da cut-off membrane.

Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus.

Polysaccharides (AJP) were prepared from Apostichopus japonicus by protease hydrolysis method. Antioxidant activity in vitro and antihyperlipidemic activity in vivo was investigated. Chemical composition analysis indicated that AJP was mainly composed of glucosamine, galactosamine, glucuronic acid, mannose, glucose, galactose and fucose, with an average molecular weight of about 36.

[The study of the secondary metabolites from fungus Paecilomyces sp].

Objective: To get active secondary metabolites from the fungus Paecilomyces sp..

Methods: The strain Paecilomyces sp.

[Study on the second metabolisms from fungus HS-1 Epicoccum spp. from the sea cucumber in Yellow Sea].

Objective: To get active metabolites from the microbes associated with sea cucumber.

Methods: Fungus was isolated from the sea cucumber, and the species was identified by molecular biology, and then was cultivated in GYP medium, and the metabolites were got by chromatography. Their structures were identified by comprehensive spectroscopic methods.

[The secondary metabolites of the mangrove endophytic fungi ZZF13 and Guignardia sp. 4382 from the South China Sea].

Objective: The secondary metabolites of the fungus ZZF13 isolated from the leaves of the mangrove sample Kandelia candel in Zhanjiang and Guignardia sp. 4382 isolated from bark of Kandelia candel (endophyte) of Mai Po, Hong Kong were studied.

Methods: The compounds were isolated by siliga gel, and their structures were identified by IR, MS and NMR.

Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats.

Aim: To investigate the antioxidant activity of chito-oligosaccharides (COSs) on pancreatic islet cells in diabetic rats induced by streptozotocin.

Methods: The antioxidant effect of COSs on pancreatic islet cells was detected under optical microscopy and with colorimetric assay and gel electrophoresis. The activities of glutathione peroxidase and superoxide dismutase, total antioxidant capacity, and content of malondialdehyde in serum and tissue slices of pancreas were examined after 60 d to determine the effect of COSs in streptozotocin-induced diabetes in rats.