[The CD133 polyclonal antibody generation and cancer stem cells identification].

Objective: To generate the cancer stem cells (CSCs) specific protein CD133 polyclonal antibody for the study of the biological characteristics of CSCs in tumor tissues and CSCs screening for the mouse model.

Methods: The extracellular peptide of the human CD133 was injected into rabbits to generate polyclonal antibody which was used for glioblastoma(GBM) Western blot and immunohistochemistry.

Results: The CD133 antiserum we made could detect both overexpressed myc-CD133 and endogenous CD133 efficiently by Western blot.

[Adherent culture of CD133+ cells in glioblastomas and expression of ADLH1].

Objective: To investigate the influence of adherent culture on the acquisition of CD133+ cells in glioblastomas and the expressions of acetaldehyde dehydrogenase 1 (ALDH1) in the undifferentiated and differentiated cells.

Methods: Adherent culture was performed with immunomagnetic bead technique in 7 glioblastoma samples to gain CD133+ cells. The cell differentiation was induced via serum medium culture.

[Preparation and identification of the Syntenin1 polyclonal antibody, a cancer metastasis related gene].

Objective: To express the GST fusion protein, GST-Syntenin1 in E. coli, and to prepare the polyclonal antibody of Syntenin1.

Methods: CDS fragment of Syntenin1 was obtained by RT-PCR from normal mouse brain and subcloned into pGEX-4T-2 to generate pGEX-4T-2-Syntenin1 recombinant.

[AQP4 expression in the brains of patients with glioblastoma and its association with brain edema].

Objective: To investigate the expression of aquaporin-4 (AQP4) in the brains of patients with glioblastoma and its association with brain edema.

Methods: Immunofluorescence cytochemistry and western blot tests were performed to detect the expression of AQP4 in the brain tumors and the adjacent tissues in 30 patients with glioblastoma. The association between AQP4 and the extent of brain edema was analysed.

[Effect of polylactic acid on the proliferation and differentiation of osteoblast-like cells].

Objective: To assess the effects of polylactic acid (PLA) on the proliferation and differentiation of UMR-106 osteoblast-like cells.

Methods: The ultrastructures of the surface of untreated and pretreated PLA films were observed under electron microscope. UMR-106 osteoblast-like cells were cultured with PLA film which was untreated or treated by some special agents together, then the cells morphology was observed, and methyl thiazolyl tetrazolium (MTT) assay and alkaline phosphatase (ALP) analysis were performed to detect the Tiny distinction between the untreated film and pretreated film was proliferation and differentiation.

[Methylation and expression analysis of p16(INK4a) and RB genes in meningiomas].

Objective: To investigate the methylation of p16(INK4a) and RB gene, and the expression of p16(INK4a) in meningiomas.

Methods: Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation of p16(INK4a) and RB in 50 cases of meningiomas, and immunostaining was performed to analyze the protein expression of p16(INK4a) in 25 of those cases.

Results: No methylation was found in the benign meningiomas, whereas methylation of p16(INK4a)or RB occurred in 6(37.