Polymorphism analysis of Chinese Theileria sergenti using allele-specific polymerase chain reaction of the major piroplasm surface protein gene.

Theileria sergenti is a tick-borne parasite found in many parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a marker for epidemiological and phylogenetic studies of benign Theileria species. In this study, Chinese species of T.

Sequence analysis of Theileria annulata surface protein in Chinese isolates.

Objective: To study the TaSP polymorphism in three Chinese isolates of Theileria annulata.

Methods: The isolates from Inner Mongolia Autonomous Region, Ningxia Hui Autonomous Region and Xinjiang Uygur Autonomous Region were cultured in RPMI 1640 medium. TaSP gene was amplified from genomic DNA extracted from schizonts using polymerase chain reaction (PCR) and sequenced.

[Prokaryotic expression of Bm86 gene of Boophilus microplus and optimization of the expression condition].

A pair of specific primers was designed based on the reported Bm86 gene of Boophilus microplus,the Bm86 gene was cloned by PCR using the plasmid pMD18-T-Bm86 as templates, and subcloned into the prokaryotic plasmid pGEX-4T-1. The recombined plasmid was transformed into E. coli BL21(DE3) and followed by expression of the protein induced by different concentration of IPTG for different time.

[Morphological comparison of two species of Theileria in sheep].

Two splenectomized sheep were infected respectively with Theileria luwenshuni and T. uilenbergi, 2 species identified by PCR. When piroplasms were found in blood smears from ears of the sheep, morphological observation on the Theileria spp.