Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a crucial role in the degradation of the low-density lipoprotein receptor (LDLR), and PCSK9 inhibition emerges as an attractive strategy for atherosclerosis management. In this study, extracellular vesicles (EVs) were engineered to nanosponges, which could efficiently adsorb and deliver PCSK9 into lysosomes for degradation. Briefly, nanosponges were engineered by modifying EVs with EGF-A/PTGFRN fusion protein (PCSK9 binding domain EGF-A from the mutant LDLR with higher affinity was fused to the C terminus of prostaglandin F2 receptor negative regulator).

Serotonin Syndrome From Duloxetine Monotherapy: A Case Report.

Serotonin syndrome is a rare adverse reaction induced by serotonergic drugs. Most instances of the syndrome occur in the context of a serotonergic drug overdose and the combined use of monoamine oxidase inhibitors and other related drugs. We report a case of serotonin syndrome caused by duloxetine alone in an elderly Asian woman and review the literature.

Real-time imaging of sulfhydryl single-stranded DNA aggregation.

The structure and functionality of biomacromolecules are often regulated by chemical bonds, however, the regulation process and underlying mechanisms have not been well understood. Here, by using in situ liquid-phase transmission electron microscopy (LP-TEM), we explored the function of disulfide bonds during the self-assembly and structural evolution of sulfhydryl single-stranded DNA (SH-ssDNA). Sulfhydryl groups could induce self-assembly of SH-ssDNA into circular DNA containing disulfide bonds (SS-cirDNA).

In Situ TEM Observation of Stagnant Liquid Layer Activation in Nanochannel.

The kinetics of mass transfer in a stagnant fluid layer next to an interface govern numerous dynamic reactions in diffusional micro/nanopores, such as catalysis, fuel cells, and chemical separation. However, the effect of the interplay between stagnant liquid and flowing fluid on the micro/nanoscopic mass transfer dynamics remains poorly understood. Here, by using liquid cell transmission electron microscopy (TEM), we directly tracked microfluid unit migration at the nanoscale.

Atomic Scale Tracking of Single Layer Oxide Formation: Self-Peeling and Phase Transition in Solution.

Liquid phase electron microscopy (TEM) is used to track the formation of In O ultrathin nanosheet in solution at atomic scale. This observation reveals that the formation of few atomic layer nanosheet goes through a complicated phase transition process from InCl 3H O to In(OH) and then to In O . Interestingly, the intermediate InCl 3H O nanosheet can grow via either layer by layer or the strain-driven enation growth from precursor solution.

The effects of a multi-disciplinary team on sleep quality assessment in mild-to-moderate Alzheimer's disease patients with sleep disorders.

Background And Aims: Sleep disturbances are a severe problem among patients with Alzheimer's disease (AD). By evaluating sleep quality in mild-to-moderate AD patients, this study aimed to assess the effects of multi-disciplinary team (MDT) in reducing the incidence of adverse reactions of AD patients. The reduction in the incidence of adverse reactions to predict multi-disciplinary team (MDT) treatment effects.

Polysaccharide isolated from Auricularia auricular-judae (Bull.) prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.

Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits. In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.