Dietary γ-Aminobutyric Acid Promotes Growth and Immune System Performance and Improves Erythropoiesis and Angiogenesis in Gibel Carp ().

This experiment aimed to investigate the effect of dietary supplementation of γ-aminobutyric acid (GABA) on the growth performance, immune response, and oxygen-transport-related factors of Gibel carp (). An eight-week culturing experiment was designed with five experimental diets, with the actual GABA content being 368 mg/kg (G1, control group), 449 mg/kg (G2), 527 mg/kg (G3), 602 mg/kg (G4), and 675 mg/kg (G5). The results showed that the level of 527 mg/kg (G3) of GABA significantly increased the specific growth rate (SGR), weight gain rate (WGR), and final body weight (FBW) of Gibel carp, while the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), and glucose (GLU) were also increased significantly.

Development a glycosylated extracellular vesicle-derived miRNA Signature for early detection of esophageal squamous cell carcinoma.

Background: Esophageal squamous cell carcinoma (ESCC) is often diagnosed at an advanced stage due to the lack of non-invasive early detection tools, which significantly impacts patient prognosis. Given that glycosylation alterations especially high sialylation and fucosylation, frequently occur during cellular malignant transformation, but their roles are not elucidated. We examined alterations in disease-specific glycosylated extracellular vesicles (EVs)-derived miRNAs in the serum of ESCC patients, evaluating their utility as diagnostic biomarkers.

Uncovering glycolysis-driven molecular subtypes in diabetic nephropathy: a WGCNA and machine learning approach for diagnostic precision.

Introduction: Diabetic nephropathy (DN) is a common diabetes-related complication with unclear underlying pathological mechanisms. Although recent studies have linked glycolysis to various pathological states, its role in DN remains largely underexplored.

Methods: In this study, the expression patterns of glycolysis-related genes (GRGs) were first analyzed using the GSE30122, GSE30528, and GSE96804  datasets, followed by an evaluation of the immune landscape in DN.

Diagnostic accuracy of gasdermin D as a biomarker for necrotizing enterocolitis: a single-center diagnostic test study.

Background: Necrotizing enterocolitis (NEC) is a devastating gastrointestinal condition mainly affecting premature infants, and gasdermin D (GSDMD) has emerged as a molecule of interest due to its pivotal role in the inflammatory process called pyroptosis in NEC pathogenesis. The aim of this study is to examine the potential of GSDMD and interleukin-1β (IL-1β) as early diagnostic biomarkers for NEC.

Methods: We examined 207 infants with clinical symptoms of NEC admitted to our neonatal intensive care unit (NICU) between December 2023 and June 2024.

Insight into the Origin of Second Harmonic Generation and Rational Design in the Metal Halide Borates.

Metal halide borates are promising candidates for high-performance nonlinear optical (NLO) applications, yet the origins of their second harmonic generation (SHG) properties remain unclear. Using atom response theory combined with density functional theory calculations, this study investigates why halogen substitution leads to distinctly different SHG responses in halide monoborates (PbBOX) versus halide pentaborates (PbBOX). We find that the SHG origins vary between these two families due to differences in the strength of the Pb-X interactions.

Pathway Polygenic Risk Scores (pPRS) for the Analysis of Gene-environment Interaction.

Unlabelled: A polygenic risk score (PRS) is used to quantify the combined disease risk of many genetic variants. For complex human traits there is interest in determining whether the PRS modifies, i.e.

Mitochondrial damage causes inflammation via cGAS-STING signaling in ketamine-induced cystitis.

Background: Mitochondrial dysfunction and damage can result in the release of mitochondrial DNA (mtDNA) into the cytoplasm, which subsequently activates the cGAS-STING pathway, promoting the onset of inflammatory diseases. Various factors, such as oxidative stress, viral infection, and drug toxicity, have been identified as inducers of mitochondrial damage. This study aims to investigate the role of mtDNA as a critical inflammatory mediator in the pathogenesis of ketamine (KET)-induced cystitis (KC) through the cGAS-STING pathway.

Automated LA-DBD-TLC-MS device for accurate detection of biogenic amines in fishery products coupled with machine learning algorithms.

A novel, compact, and automated laser ablation dielectric barrier discharge thin layer chromatography-mass spectrometry (LA-DBD-TLC-MS) device was developed for the rapid detection of biogenic amines (BAs) in fishery products. This plug-and-play system integrates thermal desorption via diode laser, DBD plasma ionization, and tandem MS detection, with key operational parameters optimized through experimental and computational methods. Utilizing nanoscale carbon black as a matrix, the device achieved a detection limit of 0.

A Study on the Function of Arginine in the Growth, Immunity, Antioxidant Activity, and Oxygen Carrying-Capacity of Juvenile Gibel Carp ().

An eight-week trial was designed to study the effects of arginine (Arg) supplemented diets on the growth, immunity, antioxidant activity, and oxygen-carrying capacity of juvenile Gibel carp (). A total of 300 fish (27.53 ± 0.

Modified Jiaoqi Powder enhances epithelial autophagy against TNF-triggered apoptosis in chronic ulcerative colitis.

Background: A vicious cycle of dysregulated intestinal epithelial cell death, intestinal barrier defect, and subsequent inflammation response is core to chronic ulcerative colitis (UC). Modified Jiaoqi Powder (MJQP), a traditional Chinese medicine formula, has been clinically applied to treat chronic relapsing and chronic persistent types of UC. Nevertheless, the underlying mechanisms of MJQP in chronic UC remains unknown.

Comparison of erector spinae plane block and transverse abdominis plane block in postoperative recovery after laparoscopic colorectal surgery: a randomized, double-blind, controlled trial.

Background: Patients experience significant postoperative pain after laparoscopic resection of colorectal cancer. Transversus abdominis plane block (TAPB) provides effective analgesia, and recent studies have also shown that erector spinae plane block (ESPB) can be used for postoperative analgesia in abdominal surgery. However, there is a lack of comparison between the two methods regarding recovery quality following laparoscopic colorectal surgery.

Unlocking the potential differences and effects of the anode and cathode regions on NO emissions during electric field-assisted aerobic composting.

Electric field-assisted aerobic composting (EAC) is a novel strategy for effectively mitigating nitrous oxide (NO) emissions, but its deeper effects require further exploration. In this study, the differences in NO emissions between the anode regions (AR) and cathode regions (CR) during EAC were evaluated. The cumulative NO emission from the compost in CR was 32.

InterPro: the protein sequence classification resource in 2025.

InterPro (https://www.ebi.ac.

Effects of Replacing Soybean Meal with Sunflower Meal or Fermented Sunflower Meal on the Growth Performance, Intestinal Microbiota, and Intestinal Health of Tilapia (GIFT, ).

A 9-week feeding trial was conducted to evaluate the effects of replacing soybean meal (SBM) with sunflower meal (SM) or fermented sunflower meal (FSM) on the growth performance, intestinal microbiota, and intestinal health of genetically improved farmed tilapia () (initial weight 6.55 ± 0.01 g).

Reversing Zearalenone Toxicity: The Role of Hydroxytyrosol in Zebrafish.

Zearalenone (ZEA) is a widely distributed mycotoxin that presents a substantial worldwide health risk to animals. Several natural compounds have shown promise in mitigating the detrimental impacts of ZEA. This study examined the detoxification potential of previously identified compounds by utilizing zebrafish embryos as a model organism.

Spatial Architecture of Single-Cell and Vasculature in Tumor Microenvironment Predicts Clinical Outcomes in Triple-Negative Breast Cancer.

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with limited treatment options, which warrants the identification of novel therapeutic targets. Deciphering nuances in the tumor microenvironment (TME) may unveil insightful links between antitumor immunity and clinical outcomes; however, such connections remain underexplored. Here, we employed a data set derived from imaging mass cytometry of 71 TNBC patient specimens at single-cell resolution and performed in-depth quantifications with a suite of multiscale computational algorithms.

Effects of Replacing Fish Meal with Rubber Seed Cake on Growth, Digestive, Antioxidant and Protein Metabolism of Juvenile Asian Red-Tailed Catfish ().

An 8-week feeding trial was conducted to assess the impact of replacing fish meal (FM) with rubber seed cake (RSC) on growth performance, digestive enzyme activity of the gut, antioxidant capacity and protein metabolism-related parameters of plasma and liver in juvenile Asian red-tailed catfish () with an average body weight of 3.21 g. Four isonitrogenous (41.

Neutrophil-to-Lymphocyte Ratio, Platelet-to-Lymphocyte Ratio, and Prognostic Nutritional Index as Prognostic Markers for Lung Carcinoma.

Lung cancer (LC) remains one of the most common malignant tumours worldwide, and assessment of its progression is important for ensuring better prognostic outcomes for patients. This study was designed to explore the prognostic role of certain indices, including the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and prognostic nutritional index (PNI) in patients with LC, to help clinics to better determine the prognosis of patients with LC, and to allow them to intervene in a timely manner. A retrospective analysis of 116 initially diagnosed patients with LC in China from 2018 to 2020 was conducted.

Unveiling the Role of Filler Characteristics in Enhancing the High-Voltage Performance of Succinonitrile-Based Solid-State Lithium-Metal Batteries.

For exploiting high-energy lithium-metal batteries, it is of utmost importance to develop electrolytes that possess exceptional ionic conductivity and an extensive electrochemical stability range. In this study, 3D PAN nanofibers and polymer electrolytes incorporating various inorganic fillers with different Lewis acid-base properties were fabricated. PAN@Al-SSE exhibits exceptional ionic conductivity (0.

ROR1 facilitates glioblastoma growth via stabilizing GRB2 to promote c-Fos expression in glioma stem cells.

Background: Glioma stem cells (GSCs) are the root cause of tumorigenesis, recurrence, and therapeutic resistance in glioblastoma (GBM), the most prevalent and lethal type of primary adult brain malignancy. The exploitation of novel methods targeting GSCs is crucial for the treatment of GBM. In this study, we investigate the function of the novel ROR1-GRB2-c-Fos axis in GSCs maintenance and GBM progression.

Laboratory-acquired infection in clinical laboratories and the incidence rate after Brucella exposure risk events: a systematic review and meta-analysis.

Background: Personnel in clinical microbiology laboratories face heightened risks of occupational infections, due to the potential for pathogenicity in clinical samples.

Aim: To summarize the characteristics of laboratory-acquired infections (LAIs) and review exposure incidents in clinical laboratories, and to conduct a meta-analysis to estimate post-exposure incidence rates and evaluate the efficacy of post-exposure prophylaxis (PEP) following Brucella exposures.

Methods: A systematic search across PubMed, Embase, Web of Science, CNKI, Wanfang, CMB, and the ABSA LAI database extracted relevant literature published from January 1, 1990, to August 31, 2023, including case reports and laboratory exposure risk events.