Publications by authors named "Meytal Dror-Levinsky"
Background: GBM is an aggressive grade 4 primary brain tumor (BT), with a 5%-13% 5-year survival. Most human GBMs manifest as immunologically "cold" tumors or "immune deserts," yet the promoting or suppressive roles of specific lymphocytes within the GBM tumor microenvironment (TME) is of considerable debate.
Methods: We used meticulous multiparametric flow cytometry (FC) to determine the lymphocytic frequencies in 102 GBMs, lower-grade gliomas, brain metastases, and nontumorous brain specimen.
Article Synopsis
- * Common methods like ELISpot and flow cytometry (FCM) were assessed for measuring vaccine efficacy; however, they often showed significant background interference affecting results.
- * The study developed a refined FCM panel incorporating early activation markers (4-1BB and CD40L) to minimize background noise, achieving much lower background expression and improving the detection of rare, antigen-specific T-cell responses.