Antitumor activity of two derivatives from 2-acylamine-1, 4-naphthoquinone in mice bearing S180 tumor.

Drugs containing a quinone moiety, such as anthracyclines, mitoxantrones and lapachol, show excellent anticancer activity. In this study, 2-butanoylamine-1,4-naphthoquinone (1) and 2-propanoylamine-1,4-naphthoquinone (2) derivatives from 2-amine-1 ,4-naphthoquinone were synthesized, and their antitumor activity in mice bearing Sarcoma 180 tumor were examined. In addition, hematology and biochemistry analyses, as well as, histopathological and morphological analyses were performed in order to evaluate the toxicological aspects of the naphthoquinones treatment.

In vivo growth-inhibition of Sarcoma 180 by an alpha-(1-->4)-glucan-beta-(1-->6)-glucan-protein complex polysaccharide obtained from Agaricus blazei Murill.

Agaricus blazei Murrill, a native mushroom of Brazil, has been widely consumed in different parts of the world due to its anticancer potential. This effect is generally attributed to its polysaccharides; however, the precise structure of these has not been fully characterized. To better understand the relationship between polysaccharide structures and antitumor activity, we investigated the effect of the intraperitoneally (i.

Efficacy of Brazilian propolis gel for the management of denture stomatitis: a pilot study.

Denture stomatitis presents as a chronic disease in denture-bearing patients, especially under maxillary prosthesis. Despite the existence of a great number of antifungal agents, treatment failure is observed frequently. Propolis, a natural bee product, possesses well-documented antifungal and anti-inflammatory activities.

Inflammatory myofibroblastic tumor of the alveolar mucosa of the mandible.

Inflammatory myofibroblastic tumor is a rare lesion composed of myofibroblastic spindle cells accompanied by inflammatory infiltrate. The objective of this paper is to report an uncommon case of inflammatory myofibroblastic tumor located in the alveolar mucosa of the mandible. A 33-year-old male presented an asymptomatic tumoral lesion, firm, pedunculated, pink-colored, covered by smooth mucosa, with focal ulceration, measuring 30x20x20 mm, located in the left posterior alveolar mucosa.

Sequential supernumerary teeth in nonsyndromic patients: report of 3 cases.

Supernumerary tooth/teeth (ST) are a well recognized clinical phenomenon defined as teeth that are additional to the normal complement. The purpose of this report was to describe 3 patients who developed multiple sequential ST. All patients were boys between 8 and 12 years old without significant medical history.

Immunoexpression of hMSH2 and hMLH1 in oral squamous cell carcinoma and its relationship to histological grades of malignancy.

Background: The aim of this study was to describe the human mutS homolog 2 (hMSH2) and human mutL homolog 1 (hMLH1) immunoexpression in different areas of oral squamous cell carcinoma (OSCC) - central/superficial (C/S) and invasive tumor front (ITF) - in an attempt to verify if the histological grade of malignancy interferes in this expression.

Methods: Forty-six samples of OSCC were analyzed histologically. Twenty-six cases (56.

Amalgam-associated oral lichenoid reaction: case report and management.

Amalgam-associated oral lichenoid reaction (AAOLR) is a rare lesion in dental practice. This article presents a case involving a striated, white, symptomatic plaque with an atrophic central area located in the left buccal mucosa and making direct contact with an amalgam restoration. Management included removing the amalgam restoration and using composite resin as a substitute.

Metallothionein immunoexpression in oral leukoplakia.

Objectives: to report the immunoexpression of metallothionein in oral leukoplakia and to correlate with histological grade and clinical localization. Leukoplakia is the most common potentially malignant lesion of the oral cavity. As the histological study of oral leukoplakia can not predict precisely the malignant transformation of this lesion, and metallothionein is a protein that has been associated with carcinogenesis, this study could be auxiliary in this histological assessment of this lesion.

P21/ WAF1 and cyclin D1 variants and oral squamous cell carcinoma.

Background: Genetic factors are known to be involved in oral squamous cell carcinoma (OSCC) development.

Method: We evaluated a possible association between CCND1 A870G and P21/WAF1 C98A polymorphisms and OSCC, as well as the impact of the genotypes on protein immunoexpression. The study group consisted of 80 individuals with histopathological diagnosis of OSCC and the control group consisted of 80 healthy individuals without oral lesions and matched by age, sex and tobacco usage.

A survey of 460 supernumerary teeth in Brazilian children and adolescents.

Objective: This study aimed to survey the demographic profile of supernumerary teeth (ST) in Brazilian children and adolescents.

Methods: A retrospective analysis was carried out of all nonsyndromic patients with ST attended at the Pediatric Oral Surgery Service of the Universidade Federal de Minas Gerais between 1995 and 2004. Diagnosis of ST was based on clinical and radiographic examination.

In vivo growth inhibition of sarcoma 180 by piperlonguminine, an alkaloid amide from the Piper species.

Many authors have already emphasized that phytochemicals from spices have biological applications. Piperlonguminine is a known alkaloid amide from peppers, including Piper divaricatum. The aim of this study was to investigate the in vitro and in vivo antitumor effects of piperlonguminine in experimental models.

EBV detection in HIV-related oral plasmablastic lymphoma.

Objectives: Plasmablastic lymphoma (PBL) of the oral cavity is an aggressive neoplasm derived from B cell, considered to be the second more common among human immunodeficiency virus (HIV)-associated malignancies. As Epstein-Barr virus (EBV) infection has been associated with this neoplasm, the aim of the present study was to assess the presence of EBV in 11 cases of oral HIV-related PBL and investigate the controversial issue of the presence of Human herpesvirus-8 (HHV-8) in these tumors.

Methods: DNA was extracted from nine cases of HIV-associated oral lymphomas, diagnosed as PBL, and genomic material was amplified by polymerase chain reaction to verify the presence of EBV.

Demographic profile of odontogenic and selected nonodontogenic cysts in a Brazilian population.

Objective: To determine the demographic profile of all histologically diagnosed odontogenic cysts (OC) and nonodontogenic cysts (nOC) over a 51-year period in the Brazilian population.

Study Design: Case records of patients with OC and nOC from the files of the Oral Pathology Service, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil, during the period of 1953-2003 were evaluated.

Results: Among 19,064 oral biopsies, 2,905 (15.

Influence of temperature on the visco-elastic properties of direct and indirect dental composite resins.

Objectives: The first aim of this study was to determine the visco-elastic properties of two direct (DiamondLite and Grandio) and two indirect (Artglass and Vita Zeta LC) dental composites over a wide range of temperatures, in order to avoid choosing a composite that would undergo sudden changes in its mechanical properties during service.

Methods: Within this objective the composites were tested immediately after fabrication or after storage at 37 degrees C, either in air or distilled water for 1 day, 7 or 90 days. During dynamic testing, the elastic modulus (E'), viscous modulus (E'') and loss tangent (tandelta) were determined using a dynamic mechanical analyzer (DMA) over a temperature range from 0 to 200 degrees C, at an approximate masticatory frequency of 1 Hz.

Tobacco and inflammation effects in immunoexpression of hMSH2 and hMLH1 in epithelium of oral mucosa.

Background: Both hMSH2 and hMLH1 are integrated in the DNA mismatch repair (MMR) system. Reduced expression of these proteins has been reported in head and neck carcinoma; however, few studies have examined the normal levels of these proteins in oral tissues or investigated factors which could interfere with this expression. The aim of this study was to detect the expression pattern of hMSH2 and hMLH1 in normal oral mucosa and to correlate this with demographic data, smoking and local inflammation.

In vitro and in vivo antitumor effect of 5-FU combined with piplartine and piperine.

It has been reported that piplartine and piperine, alkaloid/amide compounds from Piper species, show antitumor activities. In the present paper, the effects of the combination of 5-fluorouracil (5-FU) with piplartine or piperine was determined using in vitro and in vivo experimental models. Hematological and biochemical analyses, as well as histopathological and morphological analyses of the tumor and the organs, including liver, spleen and kidney, were performed in order to evaluate the toxicological aspects associated with different treatments.

Use of tetramethylbenzidine for the spectrophotometric sequential injection determination of free chlorine in waters.

A sequential injection (SI) method was developed for the spectrophotometric determination of chlorine based on the reaction between tetramethylbenzidine (TMB) and free chlorine. The advantages resulting from the use of TMB are considerable: TMB is highly selective for chlorine, it enables a fairly low quantification limit and represents a less toxic alternative to reagents such as tolidine. The use of this reaction in SI adds other advantages as it enhances the degree of automation, minimisation of reagent consumption (6.

Prevalence of oral hemangioma, vascular malformation and varix in a Brazilian population.

Hemangioma, vascular malformation and varix are benign vascular lesions, common in the head and neck regions. Studies about the prevalence of these lesions in the oral cavity are scarce. The aim of this study was to estimate the prevalence of and to obtain clinical data on oral hemangioma, vascular malformation and varix in a Brazilian population.

Retrospective analysis of peripheral odontogenic fibroma (WHO-type) in Brazilians.

Aim: The purpose of this study is to present data on a series of peripheral odontogenic fibroma, WHO (World Health Organization)-type (POF), in Brazilians and to compare the results with previously reported studies.

Methods: The study sample was made from the number of POF cases and its synonymies diagnosed from 1950 to 2004 at the Oral Pathology Service of the Federal University of Minas Gerais. The cases were reviewed in hematoxylin-eosin stain and the histological features were evaluated in accordance with the classification WHO.

GLUT-1 in oral benign vascular lesions.

Aim: To investigate the accuracy of histological diagnosis of oral hemangioma, oral vascular malformation and oral pyogenic granuloma according to immunohistochemical evaluation of the human erythrocyte-type glucose transporter protein (GLUT-1), and to observe the immunoexpression of this protein in oral varix.

Materials And Methods: Immunohistochemistry for GLUT-1 was performed in 93 histologically diagnosed cases of oral benign vascular lesions: 17 vascular malformations, 19 hemangiomas, nine varix, and 48 pyogenic granulomas. Descriptive analyses were performed.

Pro- and anti-inflammatory activities of the latex from Calotropis procera (Ait.) R.Br. are triggered by compounds fractionated by dialysis.

Objectives And Design: Previous studies have described pro- and anti-inflammatory activities displayed by the latex from Calotropis procera. This report aims to clarify these observations and shows that such activities can be segregated from the whole latex.

Methods: The latex was divided into water-soluble fractions devoid of poly-isoprene by centrifugation and dialysis and both the activities were assayed by the peritonitis model in rats.