Thin layer chromatography-direct bioautography guided isolation of β-sitosterol from the leaves of .

The aim of the study was to isolate and characterise antimicrobial agents from the leaves of using thin-layer chromatography-direct bioautography (TLC-DB). is a medicinal plant used traditionally to treat various ailments. Previous studies have shown that species of the genus contain several classes of secondary metabolites, including sterols.

Differential Preference of Burkholderia and Mesorhizobium to pH and Soil Types in the Core Cape Subregion, South Africa.

Over 760 legume species occur in the ecologically-heterogeneous Core Cape Subregion (CCR) of South Africa. This study tested whether the main symbionts of CCR legumes ( and ) are phylogenetically structured by altitude, pH and soil types. Rhizobial strains were isolated from field nodules of diverse CCR legumes and sequenced for 16S ribosomic RNA (rRNA), recombinase A () and N-acyltransferase ().