Re-isolation and revaluation of boundaries of Thecamoeba terricola Greeff, 1886 (Amoebozoa, Discosea, Thecamoebida), with description of Thecamoeba vicaria n. sp.

We studied a large species of Thecamoeba found in a glass dish with soaked moss and obtained 18s rRNA gene sequence of this organism. Morphologically, the strain was most similar to T. terricola sensu Page, but had significant differences in cell size and nuclear morphology.

Stenamoeba aeronauta n. sp., a new case of sibling species in the order Thecamoebida (Amoebozoa, Discosea).

A new speciesStenamoeba aeronautan. sp.was isolated from a culture of large thecamoebids during laboratory studies.

Thecamoeba vumurta n. sp. (Amoebozoa, Discosea, Thecamoebida) from freshwater pond sediment - a sibling species of T. striata (Penard, 1890) Schaeffer, 1926.

Until recently, it seemed that amoebae of the genus Thecamoeba could be reliably identified using light-microscopic characters. However, recent studies show the presence of sibling species in the genus Thecamoeba, which can only be reliably distinguished based on the molecular data. Here we describe a new freshwater species, Thecamoeba vumurta n.

Thecamoeba astrologa n. sp. - A new species of the genus Thecamoeba (Amoebozoa, Discosea, Thecamoebida) with an unusually polymorphic nuclear structure.

Thecamoeba astrologa n. sp. was isolated from a plant litter sample.

Vannella primoblina n. sp. - an unusual species of the genus Vannella (Amoebozoa, Discosea, Vannellida) with pronounced dorsal ridges and folds.

Amoebae of the order Vannellida (Amoebozoa, Discosea) have a fairly recognizable spatulate, fan-shaped or semi-circular outlines and a wide area of frontal hyaloplasm. They can be easily distinguished from the other groups of lobose amoebae even by light microscopy. The dorsal side of these amoebae is usually smooth and rarely bears ridges or folds, which are never numerous or regular.

Thecamoeba foliovenanda n. sp. (Amoebozoa, Discosea, Thecamoebida) - One more case of sibling species among amoebae of the genus Thecamoeba.

Until recently it seemed that amoebae of the genus Thecamoeba can be reliably identified using light-microscopic characters, like the size and shape of the cell, a characteristic pattern of folds and ridges and structure of the nucleus. However, recent molecular studies show the presence of sibling species that can be reliably distinguished based on the gene sequence data only. Here we describe a new terrestrial species, Thecamoeba foliovenanda n.

Molecular Phylogeny of Polychaos annulatum (Amoebozoa, Tubulinea, Euamoebida) Shows that Genus Polychaos Belongs to the Family Hartmannellidae.

We have obtained a sequence of the 18S rRNA gene of the species Polychaos annulatum (Penard 1902) Smirnov et Goodkov 1998 using the isolation of a single nucleus from an amoeba cell. Attempts to amplify the 18S rRNA gene from the DNA of this species by conventional PCR were not successful, so we applied the whole genome amplification of the nuclear DNA followed by NGS sequencing. The 18S rRNA gene was found among the resulting contigs.

Thecamoeba cosmophorea n. sp. (Amoebozoa, Discosea, Thecamoebida) - An example of sibling species within the genus Thecamoeba.

A new species Thecamoeba cosmophorea n. sp. (Amoebozoa, Discosea, Thecamoebida) was isolated from leaf litter collected in the surroundings of Saint-Petersburg (Russia).