Biochemical and molecular characterization of an acido-thermostable endo-chitinase from Bacillus altitudinis KA15 for industrial degradation of chitinous waste.

This paper reports the isolation and identification of an acido-thermostable chitinase (ChiA-Ba43) which was purified, from the culture liquid of Bacillus altitudinis strain KA15, and characterized. Purification of ChiA-Ba43 produced a 69.6-fold increase in the specific activity (120,000 U/mg) of the chitinase, with a yield of 51% using colloidal chitin as substrate.

Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15.

A new serine alkaline protease (designated as SAPGB) from Gracilibacillus boraciitolerans strain LO15, was produced (9000 U/mL), purified, and characterized. SAPGB has a monomer structure with a precise molecular weight of 30,285.03 kDa as learnt from matrix-assisted laser desorption/ionization-time of flight/mass spectroscopy (MALDI-TOF/MS) exploration.

Purification and biochemical characterization of a new organic solvent-tolerant chitinase from Paenibacillus timonensis strain LK-DZ15 isolated from the Djurdjura Mountains in Kabylia, Algeria.

A new extracellular chitinase (called ChiA-Pt70) was produced and purified from a newly isolated Paenibacillus timonensis strain LK-DZ15. The maximum chitinase activity recorded after 44-h of incubation at 30 °C was 11,500 U/mL. Pure enzyme was obtained after ammonium sulphate precipitation (40-70%) followed by sequential column chromatographies on fast performance liquid chromatography (FPLC) and high performance liquid chromatography (HPLC).

Evaluation of antimicrobial activity of extracts collected from Saharan desert against bacteria and filamentous fungi.

Different desert truffles, collected from Algerian Saharan soils, were identified and their capacity to produce bioactive substances with antimicrobial activity was analyzed. Based on morphological characterization using Melzer's reagent staining, the collected strains were identified as . The bioactive substances from were extracted using the following techniques: maceration with methanol and Soxhlet with dichloromethane.

Genetic polymorphisms of blood donors in Algeria through blood groups ABO, RH, and Kell.

Our study included 2465 blood donors unrelated from both sexes, originating from Msila (Algeria), at Msila Blood Transfusion Center (CTS), with the aim of performing an anthropogenetic characterization of the population of M'sila, by studying the three-erythrocyte polymorphic systems ABO, Rhesus, and Kell with their allele frequencies. This allowed us to demonstrate after a multi-varied comparative analysis through principal components analysis (PCA) and hierarchical cluster analysis (HCA) On the one hand, comparing the population of Msila to the different regions of Algeria where we found a genetic proximity with the great south of Algeria going towards the south-east of Algeria. On the other hand, the comparative analysis of Msila's population with other populations in the world based on historical, geographical and cultural profile, by building a tri-hybrid potential parenting model (North Africa, Mediterranean and Middle orient) through the three blood systems, allowed us to identify four potential parents including Egypt and Libya (North Africa) and Saudi Arabia and South Yemen (Middle East), Regarding the third strand of our tri-hybrid model, we did not find any potential parental link with the northern shore of the Mediterranean (southern Europe) despite the historical and geographical link that exists.

Antibiotic Resistance, Virulence, and Genetic Background of Community-Acquired Uropathogenic Escherichia coli from Algeria.

The aim of the study was to investigate antibiotic resistance mechanisms, virulence traits, and genetic background of 150 nonrepetitive community-acquired uropathogenic Escherichia coli (CA-UPEC) from Algeria. A rate of 46.7% of isolates was multidrug resistant.