A Cross-Sectional Serological Study to Assess the Prevalence and Risk Factors of Anaplasmosis in Dromedary Camels in Punjab, Pakistan.

Anaplasmosis is an infectious disease transmitted by ticks and caused by obligate intracellular pathogen of belonging to genus Infections of one-humped camels () and llamas () have been reported previously. The aim of this study was to investigate the seroprevalence and risk factors of anti- spp. antibodies in of the Punjab, Pakistan.

Spatial distribution of Q fever in sheep and goats of selective villages of Punjab Province, Pakistan.

This study aimed to assess the geographical distribution of Q fever in sheep and goats in different areas of Punjab, Pakistan. Three hundred blood samples of small ruminants including sheep and goats were collected from 60 villages of three districts (Okara, Kasur, and Pakpattan) of Punjab Province Pakistan and tested for the detection of anti-Coxiella burnetii antibodies using a commercial Indirect ELISA kit. Data related to sampling location, host species, gender, age, and GPS coordinates were collected for spatial analysis.

Susceptibility, Immunity, and Persistent Infection Drive Endemic Cycles of Coxiellosis on Dairy Farms.

, a zoonotic bacterium, is prevalent in dairy farms. Some cows develop a persistent infection and shed into milk and occasionally by amniotic fluid at calving. Serological diagnosis of Q fever in humans is performed by phase (Ph)-specific antibody tests; PhII antibodies usually indicate an acute infection, while the development of a chronic infection is characterised by elevated PhI antibody titres.

Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen.

Q fever, a worldwide-occurring zoonotic disease, can cause economic losses for public and veterinary health systems. Vaccines are not yet available worldwide and currently under development. In this regard, it is important to produce a whole cell antigen, with preserved structural and antigenic properties and free of chemical modifications.

Corrigendum: Sero-epidemiological study of zoonotic bacterial abortifacient agents in small ruminants.

[This corrects the article DOI: 10.3389/fvets.2023.

White-Toothed Shrews (Genus ): Potential Reservoirs for Zoonotic spp. and Arthropod-Borne Pathogens?

Three species of white-toothed shrews of the order Eulipotyphla are present in central Europe: the bicolored (), greater () and lesser () white-toothed shrews. Their precise distribution in Germany is ill-defined and little is known about them as reservoirs for zoonotic pathogens ( spp., , spp.

Macrophages inhibit Coxiella burnetii by the ACOD1-itaconate pathway for containment of Q fever.

Infection with the intracellular bacterium Coxiella (C.) burnetii can cause chronic Q fever with severe complications and limited treatment options. Here, we identify the enzyme cis-aconitate decarboxylase 1 (ACOD1 or IRG1) and its product itaconate as protective host immune pathway in Q fever.

Seroprevalence and Molecular Evidence of in Dromedary Camels of Pakistan.

Coxiellosis is a zoonosis in animals caused by . A cross-sectional study was conducted on 920 (591 female and 329 male) randomly selected camels () of different age groups from 13 districts representative of the three different ecological zones in the Province Punjab, Pakistan to determine the prevalence and associated risk factors of coxiellosis. The blood samples were collected and tested for anti- antibodies using indirect multispecies ELISA.

Sero-Epidemiology of Infection in Small Ruminants in the Eastern Region of Punjab, Pakistan.

The aim of this study was to investigate the seroprevalence of Q fever in sheep and goats in Kasur, Okara, and Pakpattan in the Punjab of Pakistan. Q fever is a widely reported zoonotic disease caused by (.) .

Serological Prevalence of and Risk Factors for Infection in Women of Punjab Province, Pakistan.

Background: Coxiella burnetii, the etiological agent of Q (query) fever, provokes abortions in ruminants and is suspected to cause adverse pregnancy outcomes in women. Infection of pregnant women is linked with high mortality and morbidity of the fetus and the mother is at high risk to acquire chronic Q fever. This research was conducted to evaluate the prevalence of Q fever in women and to detect associated risk factors in four districts of Punjab Province, Pakistan.

Zoonotic bacteria in clinically healthy goats in petting zoo settings of zoological gardens in Germany.

Goats and other small ruminants are frequently used as contact animals in petting zoo settings of zoological gardens. However, they are capable to carry a broad spectrum of zoonotic pathogens without clinical signs. In this study, we analysed the presence of different zoonotic pathogens in 300 clinically healthy goats from 14 zoological gardens in Germany.

Research Trends and Hotspots of Q Fever Research: A Bibliometric Analysis 1990-2019.

Q fever is a worldwide distributed zoonosis caused by , a Gram-negative bacterium. Despite existence of large amount of research data on the developments related to Q fever, no bibliometric analysis of this subject is available to our knowledge. Bibliometric studies are an essential resource to track scholarly trends and research output in a subject.

Inactivation Kinetics of During High-Temperature Short-Time Pasteurization of Milk.

The Gram-negative, obligate intracellular bacterium is the causative organism of the zoonosis Q fever and is known for its resistance toward various intra- and extracellular stressors. Infected ruminants such as cattle, sheep, and goats can shed the pathogen in their milk. Pasteurization of raw milk was introduced for the inactivation of and other milk-borne pathogens.

Molecular Typing of Burkholderia mallei Isolates from Equids with Glanders, India.

We collected 10 Burkholderia mallei isolates from equids in 9 districts in India during glanders outbreaks in 2013-2016. Multilocus variable-number tandem-repeat analysis showed 7 outbreak area-related genotypes. The study highlights the utility of this analysis for epidemiologically tracing of specific B.

Evaluation of the Diagnostic Potential of Recombinant Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle.

is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitivity and specificity must be developed.

Performance Analysis of Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt.

Anaplasmosis is a tick-born and potential zoonotic disease caused by () , , and . affecting bovines and camels causing significant economic losses. Camels as an integral part of the socio-economic lifestyle of nomads in semi-arid to arid ecosystems are prone to suffer from subclinical infections.

Uptake and fecal excretion of Coxiella burnetii by Ixodes ricinus and Dermacentor marginatus ticks.

Background: The bacterium Coxiella burnetii is the etiological agent of Q fever and is mainly transmitted via inhalation of infectious aerosols. DNA of C. burnetii is frequently detected in ticks, but the role of ticks as vectors in the epidemiology of this agent is still controversial.

Seroprevalence and Molecular Detection of Bovine Anaplasmosis in Egypt.

Bovine anaplasmosis is a tick-borne disease with zoonotic potential, caused by the obligate intracellular bacterium . The disease is distributed worldwide in tropical and subtropical regions. The economic losses from anaplasmosis in animals is of significant importance because it causes severe morbidity and mortality in cattle.

Serological and Molecular Investigation of in Small Ruminants and Ticks in Punjab, Pakistan.

Coxiellosis is a zoonotic disease caused by the obligate intracellular bacterium affecting the productive and reproductive capabilities of animals. This study was conducted to gain insight into the seroprevalence of coxiellosis in small ruminants in seven farms of the Punjab, Pakistan. Potential risk factors were assessed.

Quantitative Proteome Profiling of Reveals Major Metabolic and Stress Differences Under Axenic and Cell Culture Cultivation.

is the causative agent of the zoonotic disease Q fever. To date, the lipopolysaccharide (LPS) is the only defined and characterized virulence determinant of . In this study, proteome profiles of Nine Mile phase I (RSA 493, NMI) and its isogenic Nine Mile phase II (RSA 439 NMII) isolate with a deep rough LPS were compared on L-929 mouse fibroblasts and in complex (ACCM-2), and defined (ACCM-D) media.