Digital holographic microscopy and focusing methods based on image sharpness.

Digital holographic microscope allows imaging of opaque and transparent specimens without staining. A digitally recorded hologram must be reconstructed numerically at the actual depth of the object to obtain a focused image. We have developed a high-resolution digital holographic microscope for imaging amplitude and phase objects with autofocusing capability.

Real-time, auto-focusing digital holographic microscope using graphics processors.

The most significant advantage of holographic imaging is that one does not need to do focusing alignment for the scene or objects while capturing their images. To focus on a particular object recorded in a digital hologram, a post-processing on the recorded image must be performed. This post-processing, so called the reconstruction, is essentially the calculation of wave propagation in free space.