Unlocking genetic diversity for low-input systems in a changing climate through participatory characterization and GWAS of lentil landraces.

Lentils are a vital staple crop in a world seeking sustainable and secure food, but their cultivation face a threat due to yield instability, mainly arising from a lack of genetic diversity in breeding programmes. In this study, we assembled and characterized the genetic and phenotypic diversities of a collection of 106 lentil genotypes, to evaluate their breeding and cropping potential. Lentil landraces from Italy and beyond, either abandoned or still cultivated, were collected from genebanks, seed savers, universities and farmers.

Proximity-dependent biotinylation screening identifies NbHYPK as a novel interacting partner of ATG8 in plants.

Background: Autophagy is a conserved, highly-regulated catabolic process that plays important roles in growth, development and innate immunity in plants. In this study, we compared the rate of autophagy induction in Nicotiana benthamiana plants infected with Tobacco mosaic virus or the TMV 24A + UPD mutant variant, which replicates at a faster rate and induces more severe symptoms. Using a BirA* tag and proximity-dependent biotin identification (BioID) analysis, we identified host proteins that interact with the core autophagy protein, ATG8 in TMV 24A + UPD infected plants.

In planta proximity-dependent biotin identification (BioID) identifies a TMV replication co-chaperone NbSGT1 in the vicinity of 126 kDa replicase.

Tobacco mosaic virus (TMV) is a positive, single-stranded RNA virus. It encodes two replicases (126 kDa and 183 kDa), a movement protein and a coat protein. These proteins interact with host proteins for successful infection.