Spatial monitoring of hydrolysis in a plug-flow bioreactor: a support for flexible operation?

Hydrolysis at changing hydraulic retention time, recirculation, bedding straw content in the feed, bioaugmentation and the impact of those changes on gradient formation in the liquid phase in plug-flow reactors (PFRs) was examined. The pH-value, conductivity and oxidation-reduction potential (ORP) were monitored at three spots along the PFRs to study potential correlations to process performance during a total process time of 123 weeks. The on-line monitoring showed good correlations to acidogenesis: namely, the pH and ORP to the acidification, to butyric (and lactic) acid concentration and to the acid yield.

Determining weathering-induced heterogeneous oxidation profiles of polyethylene, polypropylene and polystyrene using laser-induced breakdown spectroscopy.

Weathering-induced polymer degradation is typically heterogeneous which plays an integral part in fragmentation. Despite that, the current selection of techniques to investigate such heterogeneities, especially beneath the sample surface, is sparse. We introduce Laser-induced Breakdown Spectroscopy (LIBS) as an analytical tool and evaluate its performance for depth profiling.

Plug-flow hydrolysis with lignocellulosic residues: effect of hydraulic retention time and thin-sludge recirculation.

Background: Two parallel plug-flow reactors were successfully applied as a hydrolysis stage for the anaerobic pre-digestion of maize silage and recalcitrant bedding straw (30% and 66% w/w) under variations of the hydraulic retention time (HRT) and thin-sludge recirculation.

Results: The study proved that the hydrolysis rate profits from shorter HRTs while the hydrolysis yield remained similar and was limited by a low pH-value with values of 264-310 and 180-200 g kg for 30% and 66% of bedding straw correspondingly. Longer HRT led to metabolite accumulation, significantly increased gas production, a higher acid production rate and a 10-18% higher acid yield of 78 g kg for 66% of straw.

D-Transposition of the great arteries with restrictive foramen ovale in the fetus: the dilemma of predicting the need for postnatal urgent balloon atrial septostomy.

Objective: Restrictive foramen ovale (FO) in dextro-transposition of the great arteries (d-TGA) with intact ventricular septum may lead to severe life-threatening hypoxia within the first hours of life, making urgent balloon atrial septostomy (BAS) inevitable. Reliable prenatal prediction of restrictive FO is crucial in these cases. However, current prenatal echocardiographic markers show low predictive value, and prenatal prediction often fails with fatal consequences for a subset of newborns.

Single-center outcome analysis of 46 fetuses with megacystis after intrauterine vesico-amniotic shunting with the Somatex®intrauterine shunt.

Objectives: To assess the spectrum of underlying pathologies, the intrauterine course and postnatal outcome of 46 fetuses with megacystis that underwent intrauterine vesico-amniotic shunting (VAS) with the Somatex® shunt in a single center.

Methods: Retrospective analysis of 46 fetuses with megacystis that underwent VAS either up to 14 + 0 weeks (early VAS), between 14 + 1 and 17 + 0 weeks (intermediate VAS) or after 17 + 0 weeks of gestation (late VAS) in a single tertiary referral center. Intrauterine course, underlying pathology and postnatal outcome were assessed and correlated with the underlying pathology and gestational age at first VAS.

Influence of Sample Wetting Method on ESC-Behavior of PMMA under Dynamic Fatigue Crack Propagation.

Environmental stress cracking (ESC) is one of the most prominent failure mechanisms for polymer components. The high sensitivity of plastics in regard to environmental influences has always meant that plastics as materials have been viewed very critically in outdoor applications. Recently, the massive occurrence of microplastics in the environment means that questions about the long-term stability of plastic parts and the studies of plastic fragmentation are of great scientific interest.

Degradation of low-density polyethylene to nanoplastic particles by accelerated weathering.

When plastics enter the environment, they are exposed to abiotic and biotic impacts, resulting in degradation and the formation of micro- and nanoplastic. Microplastic is ubiquitous in every environmental compartment. Nevertheless, the underlying degradation processes are not yet fully understood.

Validation of a Novel Boxing Monitoring System to Detect and Analyse the Centre of Pressure Movement on the Boxer's Fist.

The examination of force distribution and centre of pressure (CoP) displacement is a common method to analyse motion, load, and load distribution in biomechanical research. In contrast to gait analysis, the force progression in boxing punches is a new field of investigation. The centre of pressure displacement and distribution of forces on the surface of the fist during a boxing punch is of great interest and crucial to understanding the effect of the punch on the biological structures of the hand as well as the technical biomechanical aspects of the punching action.

Application of a Validated Innovative Smart Wearable for Performance Analysis by Experienced and Non-Experienced Athletes in Boxing.

An athlete's sporting performance depends to a large extent on the technical execution of the athletic motion in order to achieve maximum effectiveness in physical performance. Performance analysis provides an important means of classifying and quantifying athletic prowess in terms of the significant performance aspects of the sport to provide objective feedback. This study aimed to analyze technical execution in terms of punch trajectory, force, velocity and time, considering the expert-novice paradigm by investigating the technical execution of 31 experienced and non-experienced athletes for the four main punching techniques of the cross, jab, uppercut and hook strike.

Impact of Enzymatic Degradation on the Material Properties of Poly(Ethylene Terephthalate).

With macroscopic litter and its degradation into secondary microplastic as a major source of environmental pollution, one key challenge is understanding the pathways from macro- to microplastic by abiotic and biotic environmental impact. So far, little is known about the impact of biota on material properties. This study focuses on recycled, bottle-grade poly(ethylene terephthalate) (r-PET) and the degrading enzyme PETase from .

Validation of a Unique Boxing Monitoring System.

Much development work and scientific research has been conducted in recent years in the field of detecting human activity and the measurement of biomechanical performance parameters using portable sensor technologies, so-called wearable systems. Despite the fact that boxers participating in one of the most vigorous and complex disciplines of all sports, it is one of the disciplines where no noteworthy, advanced performance analytic tools are used for training or for competition purposes worldwide. This research aimed to develop and validate a comprehensive punch performance sensor system for the measurement and analysis of biomechanical parameters in the sport of boxing.

A versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation.

Accumulation of plastic and subsequent microplastic is a major environmental challenge. With the discovery of potent polyethylene terephthalate (PET)-degrading enzymes, a new perspective arose for environmental decomposition as well as technical recycling. To explore the enormous diversity of potential PET-degrading enzymes in nature and also to conveniently employ techniques like protein engineering and directed evolution, a fast and reliable assay platform is needed.

Reconstructing the Environmental Degradation of Polystyrene by Accelerated Weathering.

The fragmentation of macro- into microplastics (MP) is the main source of MP in the environment. Nevertheless, knowledge about degradation mechanisms, changes in chemical composition, morphology, and residence times is still limited. Here, we present a long-term accelerated weathering study on polystyrene (PS) tensile bars and MP particles using simulated solar radiation and mechanical stress.

Gender Identities in Organized Sports-Athletes' Experiences and Organizational Strategies of Inclusion.

In relation to conceptualizing sports, beliefs about sex binary and male hegemony are dominant. To match these assumptions and provide level playing fields, sport systems are based on sex-segregation. Thus, people who do not fit into or reject fitting into sex categories are hindered from participating in sports, particularly organized sports.

Properties of Styrene-Maleic Anhydride Copolymer Compatibilized Polyamide 66/Poly (Phenylene Ether) Blends: Effect of Maleic Anhydride Concentration and Copolymer Content.

Polyamide 66 (PA66)/poly (2,6-dimethyl-1,4-phenylene ether) (PPE) blends with a ratio of 50/50 (w/w) were produced by a twin-screw compounder. The immiscible blends were compatibilized using two different styrene-maleic anhydride copolymers (SMA) with a low (SMA) and a high (SMA) maleic anhydride (MA) concentration of 8 and 25 wt%, respectively. Furthermore, the SMA content was varied from 0 to 10 wt%.

Severe Pulmonary Stenosis or Atresia with Intact Ventricular Septum in the Fetus: The Natural History.

Purpose: To assess the intrauterine course, the outcome, and to establish a new prenatal echocardiographic scoring system to predict biventricular (BV) versus univentricular (UV) outcome of fetuses with severe pulmonary stenosis or atresia with intact ventricular septum (PSAIVS).

Methods: All cases of PSAIVS diagnosed prenatally over a period of 14years were retrospectively collected in 2 tertiary referral centers.

Results: Forty-nine fetuses with PSIVS (n = 11) or PAIVS (n = 38) were identified prenatally.

Prenatal diagnosis, associated findings and postnatal outcome of fetuses with double outlet right ventricle (DORV) in a single center.

Objective To assess the spectrum of associated anomalies, the intrauterine course, postnatal outcome and management of fetuses with double outlet right ventricle (DORV). Methods All cases of DORV diagnosed prenatally over a period of 8 years were retrospectively collected in a single tertiary referral center. All additional prenatal findings were assessed and correlated with the outcome.

On the critical role of the substrate: the adsorption behaviour of tetrabenzoporphyrins on different metal surfaces.

The adsorption behaviour of 2H-5,10,15,20-tetraphenyltetrabenzoporphyrin (2HTPTBP) on different metal surfaces, i.e., Ag(111), Cu(111), Cu(110), and Cu(110)-(2 × 1)O was investigated by scanning tunnelling microscopy at room temperature.

Phenyl Esters Are Potent Inhibitors of Caseinolytic Protease P and Reveal a Stereogenic Switch for Deoligomerization.

Caseinolytic protease P (ClpP) represents a central bacterial degradation machinery that is involved in cell homeostasis and pathogenicity. The functional role of ClpP has been studied by genetic knockouts and through the use of beta-lactones, which remain the only specific inhibitors of ClpP discovered to date. Beta-lactones have served as chemical tools to manipulate ClpP in several organisms; however, their potency, selectivity and stability is limited.

Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control.

Cells respond to DNA damage by activating cell cycle checkpoints to delay proliferation and facilitate DNA repair. Here, to uncover new checkpoint regulators, we perform RNA interference screening targeting genes involved in ubiquitylation processes. We show that the F-box protein cyclin F plays an important role in checkpoint control following ionizing radiation.

Modelling antibiotic and cytotoxic isoquinoline effects in Staphylococcus aureus, Staphylococcus epidermidis and mammalian cells.

Isoquinolines (IQs) are natural substances with an antibiotic potential we aim to optimize. Specifically, IQ-238 is a synthetic analog of the novel-type N,C-coupled naphthylisoquinoline (NIQ) alkaloid ancisheynine. Recently, we developed and tested other IQs such as IQ-143.

Synergism in the effect of prior jasmonic acid application on herbivore-induced volatile emission by Lima bean plants: transcription of a monoterpene synthase gene and volatile emission.

Jasmonic acid (JA) plays a central role in induced plant defence e.g. by regulating the biosynthesis of herbivore-induced plant volatiles that mediate the attraction of natural enemies of herbivores.

Effect of sequential induction by Mamestra brassicae L. and Tetranychus urticae Koch on lima bean plant indirect defense.

Attack by multiple herbivores often leads to modification of induced plant defenses compared to single herbivory, yet little is known about the effects on induced indirect plant defense. Here, we investigated the effect of sequential induction of plant defense by Mamestra brassicae caterpillar oral secretion and an infestation by Tetranychus urticae spider mites on the expression of indirect plant defense in Lima bean plants. The effect on indirect defense was assessed using behavior assays with the specialist predatory mite Phytoseiulus persimilis in an olfactometer, headspace analysis of 11 major herbivore-induced plant volatiles (HIPVs) including (E)-β-ocimene, and transcript levels of the corresponding gene Phaseolus lunatus (E)-β-ocimene synthase (PlOS).

Reciprocal crosstalk between jasmonate and salicylate defence-signalling pathways modulates plant volatile emission and herbivore host-selection behaviour.

The jasmonic acid (JA) and salicylic acid (SA) signalling pathways, which mediate induced plant defence responses, can express negative crosstalk. Limited knowledge is available on the effects of this crosstalk on host-plant selection behaviour of herbivores. We report on temporal and dosage effects of such crosstalk on host preference and oviposition-site selection behaviour of the herbivorous spider mite Tetranychus urticae towards Lima bean (Phaseolus lunatus) plants, including underlying mechanisms.

Amythiamicin D and related thiopeptides as inhibitors of the bacterial elongation factor EF-Tu: modification of the amino acid at carbon atom C2 of ring C dramatically influences activity.

Three analogues of amythiamicin D, which differ in the substitution pattern at the methine group adjacent to C2 of the thiazole ring C, were prepared by de novo total synthesis. In amythiamicin D, this carbon atom is (S)-isopropyl substituted. Two of the new analogues carry a hydroxymethyl in place of the isopropyl group, one at an S- (compound 3 a) and the other at an R-configured stereogenic center (3 b).