[Features of severe surgical infections of soft tissues].

Aim: To analyze clinical and microbiological examinations in severe soft tissues infections and their significance in predictors of outcomes.

Material And Methods: Treatment of 19 patients with severe suppurative infection and suspected non-clostridial anaerobic flora with different causes of soft tissues infection was analyzed. All patients were treated in N.

[Taxonomic characteristics and mixed communities of wound infection agents in patients of reanimation and surgical departments of a hospital].

Aim: Study of taxonomical structure ofwound infection agents, prevalence of mixes, and detection of character of their possible connection with the results of various microorganisms population interaction in septic wounds.

Materials And Methods: A microbiological study of material from patients with wound infection (WI), 582 of those were cured in reanimation and intensive therapy departments (RITD; group 1) and 1455 - in surgical departments (SD; group 2), was performed. Taxonomic membership and ability to coexist was determined in 4129 microorganisms strains.

[Microbiological diagnosis of gas gangrene caused by Clostridium septicum (a clinical case)].

Microscopy of gram-stained impression smears is used for the rapid diagnosis of microorganisms in the wound. The shin tissues of patient P. with suspected gas gangrene of lower extremity soft tissues were microscopically found to have gram-positive spore-forming bacteria that were morphologically similar to C.

[Mono- and associated microflora during wound infection in patients with burns].

Aim: To study etiologic role of microorganisms during wound infection and elucidation of their populations interaction in patientswith burns.

Materials And Methods: 2137 patients with wound infection treated in burn center were studied. 3179 samples of wound exudates and 6501 strains of microorganisms were studied.

[Influence of various pectins on production of staphylococcal enterotoxins types A and B].

Experimental in vitro study of influence of 2% solution of pectins (red beet, apple, citrus, manufactured by "Vitaline" company, citrus high- and low-etherified pectins, manufactured by "Hercules" company, Unipectine OB 700, and biologically active supplement "Pecto") on growth of staphylococci and production by them of type A and B enterotoxins was performed. It was shown that red beet, citrus high- and low-etherified pectins, as well as biologically active supplement "Pecto" render bactericidal effect on staphylococci and inhibit synthesis of types A and B staphylococcal enterotoxins. Citrus pectin "Vitaline" and Unipectine OB 700 don't have such influence.

[Antibiotic susceptibility of the bacterial strains isolated at the emergency hospital].

Comparative evaluation of the incidence of the drug resistant pathogens at the patients with sepsis was performed. High ratio of the resistnt strain was shown and the most potent drugs were estimated. The investigation results demonstrates the necessity to improve diagnostic quality control.

[Prophylaxis of wound infection with immobilized antibacterial drugs].

New drug ambipor that is an immobilized antibacterial agent with local prolonged therapeutic activity was developed for prophylaxis of wound suppuration. In ambipor A drugs gentamicin, dioxidin, kephzol and riphampicin are immobilazed, in iodopor - iodine. Experimental studies on 455 white rats showed that ambipor prevents suppuration, abscess formation around ligatures, improve regeneration and fibrillogenesis.

[Immunologic methods in the diagnostics and treatment of nosocomial cases of pneumonia].

The publications on the etiology, bacteriological and immunochemical diagnostics of pneumonia, as well as on the role of immunotherapy and prophylaxis in the treatment of this disease, are analyzed. The importance of immunological methods in the diagnostics of pneumonia is pointed out. Approaches to the immunotherapy and immunoprophylaxis of pneumonia are updated.

[Role of pectins in the complex treatment in patients in neurointensive care units].

Clinical and laboratory estimation of the efficacy of pectins in complex treatment of patients with intracranial hematoma was performed. It was shown that in the group of the patients treated with pectins vs the control group development of pyo-inflammatory infections was less frequent, the indices of the immunity status improved and a more rapid decrease of the intoxication and a more rapid normalization of the composition of various biotopes in the patients were observed.

[Bacteriological monitoring of infective agents of purulent septic diseases in an emergency hospital].

During the period of 1995-2001 bacteriological examination of 13,842 patients with purulent septic diseases (PSD) was carried out. The statistical processing of data revealed that equalization of the dynamic rows of intensive and extensive values characterizing the number of patients with PSD caused by etiologically important infective agents made it possible to evaluate the reliability of information. A trend to increased etiological importance of the genera Pseudomonas, Escherichia and Klebsiella was established.

[Topical pectins for the treatment of burn wounds].

The results of the burned wounds treatment with 1-2% solution of apple or beet pectins were analysed. The control results were examined at the same patients with symmetrical wounds treated by chlorhexidine, levomecol, furacillin liniment. Clinical evaluation demonstrated good tolerability of the treatment regime, absence of side effects and complications, inhibition of inflammation, acceleration of epithelisation of the burns II-IIIA class, allowed to diminish the preliminary period before autodermoplastic operation (burns of IIIB class), diminished microbial dissemination and dissemination with Pseudomonas aeruginosa.

[Comparative population study of the etiological role of causative agents of purulent inflammatory diseases and serum antibodies to antigens of opportunistic microorganisms].

The comparative study of the relationship between the levels of serum antibodies to the antigens of opportunistic microorganisms of 5 genera (Pseudomonas, Proteus, Staphylococcus, Klebsiella, Escherichia) and the microbial status was carried out. A total of 854 patients from 10 profile departments of a surgical hospital were examined. Population analysis and statistical methods of processing the results of the examination of 353 practically healthy subjects and 268 blood and plasma donors permitted the norms for the levels of specific antimicrobial antibodies (decreased, normal, elevated levels) were established.

[Dynamics of antibiotic resistance of purulent septic pathogens at an emergency facility].

The predominant pathogens at the emergency medicine hospital were analysed and its resistance to antibiotics was investigated in dynamics. The susceptibility of the pathogens was analysed by the method of dynamic equalibration. Statistically significant reduction of resistant bacteria isolation was demonstrated for 2 species of 7--that is S.

[Oral administration of pectins for prophylaxis and treatment of purulent septic complications in patients with burns].

Clinical and laboratory evaluation of pectins application efficacy demonstrated that the patients treated with pectines has lower frequency of bacteriemia, intoxication, infectious complications and lethality. Pectins use per os resulted by acceleration of burns wounds healing and by lower microbial dissemination. Frequency of coagulase-positive staphylococci, streptococci and enterococci isolation was also lower in the treated group of patients when compared to the control group.

[Efficacy of bacteriophages in complex treatment of patients with burn wounds].

Results of clinical and laboratory evaluation of the treatment with pyobacteriophage in tablets of the patients with burn wounds are presented. It was shown that phagotherapy provided more rapid cure of pyoseptic complications, temperature normalization, wounds purification and lower lethality Bacteriological analysis of wound secretions revealed that after the treatment staphylococci and streptococci were cultured 2 times rarely, Proteus spp. Were isolated 1.

[Possibility of using microbiological data in epidemiological analysis of hospital pneumonias].

A total of 528 patients with nosocomial pneumonias were examined by bacteriological methods during 4 years. The data on the patients and on 2468 bacterial strains isolated from them were computer processed using original software "Hospital Infection". Special attention was paid to hospital strains of pneumonia agents of the same taxonomic position with identical markers of antibacterial resistance.

[The use of data on the composition and drug resistance of the causative agents of suppuration in a retrospective analysis of the epidemiological situation in a hospital].

The analysis of bacterial 16,530 strains, dynamically isolated from 6,157 patients with purulent septic processes (PSP) in surgical, traumatological, burn, toxicological and resuscitation departments, was made. The computer processing of data on the spread of the causative agents of PSP, depending on their taxonomic classification and drug resistance spectra, was carried out, which made in possible to obtain information on the outbreaks of hospital infections. Correlation of the number of PSP cases and the spread of hospital resistovars was analyzed.

[Etiotropic antibiotic therapy and immunotherapy of patients with pyo-inflammatory processes].

A 3-year experience was reviewed in the treatment of patients with pyoinflammatory processes (PIP) which developed during the postoperative period in 387 persons with closed injury and multiple penetrating wounds of the chest and stomach complicated by massive hemorrhage. When the PIP was localized in the abdominal cavity, enterobacteria and bacteroides were the main pathogens: 49.4 +/- 2.

[Tasks and possibilities of bacteriological laboratories in computer-assisted control of hospital infections].

The authors discuss the organization of epidemiological surveillance of hospital infections, carried out by bacteriological laboratories. The principal tasks of epidemiological surveillance of pyoseptic infections in surgical hospitals are formulated. The potentialities of using the data of computer monitoring of nosocomial infections are discussed.

[Diagnosis of chlamydia and gardnerella infection in patients with acute gynecological diseases].

Chlamydial infection was diagnosed by indirect immunofluorescence in 26.3% and Gardnerella infection in 28.7% of patients with acute gynecological diseases.

[Antimicrobial properties of pectins and their effects on antibiotics].

The influence of food fibres and plant proteins on microorganisms, bacteriophages, antibiotics and penicillinase was studied in vitro. It was shown that pectin was the only agent that had a bactericidal effect on the most widely distributed pathogenic and opportunistic microorganisms and did not influence indigenic microflora. High concentrations of pectin (> 2 per cent) had an inactivating effect on therapeutic bacteriophages.