Screening of Hub Genes and Therapeutic Drugs in Cervical Cancer Using Integrated Bioinformatics Analysis.

Cervical cancer (CC) is one of the most common female malignancies globally. The current study aimed to identify novel hub genes associated with traditional Chinese herbs and investigate their underlying mechanisms using bioinformatics analysis combined with experimental verification. Expression profiling of 22 samples was obtained from the GEO database.

Sec24C Participates in Cuticular Wax Transport by Facilitating Plasma Membrane Localization of ABCG5.

Cuticular waxes synthesised in the endoplasmic reticulum of epidermal cells must be exported to the outer surface of the epidermis to fulfil their barrier function. Beyond transmembrane trafficking mediated by ABC transporters, little is known about the movement of wax molecules. In this study, we characterise a mutant named sugar-associated vitrified 1 (sav1), which exhibits a vitrified phenotype and displays a reduced root length when cultivated on sugar-free medium.

Association between homologous recombination deficiency status and carboplatin treatment response in early triple-negative breast cancer.

Background: The aim of this study was to assess homologous recombination deficiency (HRD) status and its correlation with carboplatin treatment response in early triple-negative breast cancer (TNBC) patients.

Methods: Tumor tissues from 225 consecutive TNBC patients were evaluated with an HRD panel and homologous recombination-related (HRR) gene expression data. HRD positivity was defined as a high HRD score and/or BRCA1/2 pathogenic or likely pathogenic mutation.

Association of tumor immune infiltration and prognosis with homologous recombination repair genes mutations in early triple-negative breast cancer.

The aim of this study was to evaluate the mutation spectrum of homologous recombination repair (HRR) genes and its association with tumor immune infiltration and prognosis in triple-negative breast cancer (TNBC). TNBC patients (434 patients from Ruijin cohort) were evaluated with targeted next-generating sequencing for mutations in HRR genes. The frequencies of mutations were compared with public reference cohorts (320 TNBC patients from METABRIC, 105 from TCGA, and 225 from MSKCC 2018).

Evaluation of the Efficacy of OSU-2S in the Treatment of Non-Small-Cell Lung Cancer and Screening of Potential Targets of Action.

(1) Background: OSU-2S is a derivative of FTY720 and exhibits significant inhibitory effects on various cancer cells. There is currently no research on the mechanism of the impact of OSU-2S on NSCLC development. We analysed and validated the hub genes and pharmacodynamic effects of OSU-2S to treat NSCLC.

Antitumor effects of a novel photosensitizer-mediated photodynamic therapy and its influence on the cell transcriptome.

Photodynamic therapy (PDT) is a promising cancer treatment. This study investigated the antitumor effects and mechanisms of a novel photosensitizer meso-5-[ρ-diethylene triamine pentaacetic acid-aminophenyl]-10,15,20-triphenyl-porphyrin (DTP) mediated PDT (DTP-PDT). Cell viability, reactive oxygen species (ROS), and apoptosis were measured with a Cell Counting Kit-8 assay, DCFH-DA fluorescent probe, and Hoechst staining, respectively.

[Total polyphenols of Cydonia oblonga inhibited proliferation and migration of renal cancer cells by PI3K/Akt/mTOR pathway].

The mechanism of total polyphenols of Cydonia oblonga Miller(TPCOM) against kidney cancer was elucidated through a combination of network pharmacology, bioinformatics, and experimental verification. The active polyphenolic compounds from C. oblonga were screened by network pharmacological techniques and kidney cancer-related targets were collected through the database.

Pharmacological Effects of FTY720 and its Derivatives.

FTY720 is an analog of sphingosine-1-phosphate (S1P) derived from the ascomycete Cordyceps sinensis. As a new immunosuppressant, FTY720 is widely used to treat multiple sclerosis. FTY720 binds to the S1P receptor after phosphorylation, thereby exerting immunosuppressive effects.

Mechanisms underlying therapeutic resistance of tyrosine kinase inhibitors in chronic myeloid leukemia.

Chronic myeloid leukemia (CML) is a malignant clonal disease involving hematopoietic stem cells that is characterized by myeloid cell proliferation in bone marrow and peripheral blood, and the presence of the Philadelphia (Ph) chromosome with BCR-ABL fusion gene. Treatment of CML has dramatically improved since the advent of tyrosine kinase inhibitors (TKI). However, there are a small subset of CML patients who develop resistance to TKI.

Repetition velocity as a measure of loading intensity in the free weight and Smith machine Bulgarian split squat.

This study investigated the grouped and individualized load-velocity profile (GLVP ILVP) in Bulgarian split squat using Smith machine and free weight. Seventy five recreational male lifters completed two incremental loading tests of Bulgarian split squat. Mean velocity was measured by a linear-position transducer (GymAware).

TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation.

Background: Lung adenocarcinoma (LUAD) has high morbidity and is prone to recurrence. TIMELESS (TIM), which regulates circadian rhythms in Drosophila, is highly expressed in various tumors. Its role in LUAD has gained attention, but the detailed function and mechanism have not been clarified completely at present.

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl accumulation.

Background: Overaccumulation of chloride (Cl) when plants suffer NaCl causes cell damage and death, and is regulated by Cl channel protein (CLC). Apple roots are very sensitive to Cl, but information associated with CLC is limited in apple crop that widely cultivated in the world.

Results: We identified 9 CLCs from the apple genome and divided them into two subclasses.

Optimal velocity loss threshold for inducing post activation potentiation in track and field athletes.

The aim of this study was to determine the optimal velocity loss (VL) threshold that maximises the post activation potentiation (PAP) stimulus for achieving larger and more consistent performance gains in track and field athletes. Twenty-two athletes from athletics participated in four back squat PAP tests with four different VL threshold (5%, 10%, 15% and 20% VL) at an intensity of 85%1RM. Countermovement jump (CMJ) height, power, and momentum were assessed before, and 10 s, 4, 8, 12, 16 minutes after the PAP condition.

Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway.

We identified the effective components and the underlying mechanisms of Quince (Cydonia oblonga Mill, COM) extract against atherosclerosis. The effective components of COM extract were identified with UHPLC-Q-TOF-MS/MS. Network pharmacology was performed.

A Network Pharmacology-Based Study on the Mechanism of Dibutyl Phthalate of L. against Alzheimer's Disease through the AKT/GSK-3 Pathway.

Background: L. (OBL) is mainly used to treat neurological diseases in China. The preliminary work of this group showed that OBL improves cognitive impairment in Alzheimer's disease (AD).

HS Enhanced the Tolerance of to Alkaline Salt Stress through the Expression of Genes Related to Sulfur-Containing Compounds and the Cell Wall in Roots.

Malus is an economically important plant that is widely cultivated worldwide, but it often encounters saline-alkali stress. The composition of saline-alkali land is a variety of salt and alkali mixed with the formation of alkaline salt. Hydrogen sulfide (HS) has been reported to have positive effects on plant responses to abiotic stresses.

Influence of sleep disruption on inflammatory bowel disease and changes in circadian rhythm genes.

According to clinical investigations, sleep disruption (SD) can influence the immune system and cause inflammatory bowel disease (IBD). However, the detailed effects of sleep on IBD development and progression have not been clarified. Here, we used dextran sulfate sodium (DSS) to induce colitis in mice, and then interfered with SD (day-time 8:00 a.

UHPLC-Q-TOF-MS/MS and Network Pharmacology Analysis to Reveal Quality Markers of Xinjiang Mill. for Antiatherosclerosis.

Mill. (COM), mature fruit of genus Rosaceae, is consumed as a kind of traditional Chinese medicinal herb. Previous studies have shown that the components in COM extract have antioxidant, anti-inflammatory, blood pressure-lowering, blood lipid-lowering, antithrombotic, and other biological activities.

Comparison of Physiological and Perceptional Responses to 5-m Forward, Forward-Backward, and Lateral Shuttle Running.

Purpose: The aim of this study was to investigate the physiological and perceptional responses to forward, forward-backward, and lateral shuttle running.

Methods: Twenty-four eligible male subjects performed a maximal oxygen uptake (VO) test and three directional modes (i.e.

Effects of velocity based training vs. traditional 1RM percentage-based training on improving strength, jump, linear sprint and change of direction speed performance: A Systematic review with meta-analysis.

Background: There has been a surge of interest on velocity-based training (VBT) in recent years. However, it remains unclear whether VBT is more effective in improving strength, jump, linear sprint and change of direction speed (CODs) than the traditional 1RM percentage-based training (PBT).

Objectives: To compare the training effects in VBT vs.

Enteromorpha prolifera polysaccharide prevents high- fat diet-induced obesity in hamsters: A NMR-based metabolomic evaluation.

Enteromorpha prolifera polysaccharide (EP) has been shown to exhibit hypolipidemic and hypoglycemic activities in various experimental models. Here, an H-NMR-based metabolomic study was conducted to explore the regulatory effects of EP on serum metabolic changes in obese hamsters. High-fat diet (HFD)-fed hamsters were orally administrated with EP (300, 450, or 600 mg/kg) once daily for 12 weeks.

From Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli.

Excessive cadmium (Cd) damages plants by causing cell death. The present study discusses the function of natural resistance-associated macrophage protein (NRAMP) on cell death caused by Cd in . was isolated from roots, and its protein was located in the cell membrane as a transmembrane protein characterized by hydrophobicity.

Polysaccharides from Enteromorpha prolifera protect against carbon tetrachloride-induced acute liver injury in mice via activation of Nrf2/HO-1 signaling, and suppression of oxidative stress, inflammation and apoptosis.

To investigate whether polysaccharides from Enteromorpha prolifera (EPP) could protect against acute hepatic injury induced by CCl, ICR mice were pretreated with EPP (150, 300, and 450 mg kg) and silymarin (100 mg kg) for 28 days before CCl induction. Pretreatment with EPP attenuated CCl-induced elevated serum transaminase activities and histopathological alterations in the liver. In addition, EPP prevented CCl-induced reduction of protein levels of phosphorylated nuclear factor E2-related factor 2 (p-Nrf2)/Nrf2, heme oxygenase-1 (HO-1), and mRNA levels of NADPH quinineoxidoreductase-1 (NQO-1), which, in turn, reduced hepatic oxidative stress injury.