WSB1/2 target chromatin-bound lysine-methylated RelA for proteasomal degradation and NF-κB termination.

Proteasome-mediated degradation of chromatin-bound NF-κB is critical in terminating the transcription of pro-inflammatory genes and can be triggered by Set9-mediated lysine methylation of the RelA subunit. However, the E3 ligase targeting methylated RelA remains unknown. Here, we find that two structurally similar substrate-recognizing components of Cullin-RING E3 ligases, WSB1 and WSB2, can recognize chromatin-bound methylated RelA for polyubiquitination and proteasomal degradation.

Embedding Red Emitters in the NbO-Type Metal-Organic Frameworks for Highly Sensitive Luminescence Thermometry over Tunable Temperature Range.

The intrinsic advantages of metal-organic frameworks (MOFs), including extraordinarily high porosities, tailorable architectures, and diverse functional sites, make the MOFs platforms for multifunctional materials. In this study, we synthesized two kinds of isostructural NbO-type Zn-based MOFs, where two structurally similar tetracarboxylate ligands, 5,5'-(pyrazine-2,5-diyl)diisophthalic acid (HPZDDI) and 5,5'-(pyridine-2,5-diyl)diisophthalic acid (HPDDI), with pyridine or pyrazine moieties, were employed as the organic linkers. By embedding the red-emitting cationic units of pyridinium hemicyanine dye 4-[p-(dimethylamino)styryl]-1-methylpyridinium (DSM) and trivalent europium ion (Eu), two types of composites, DSM@ZnPZDDI and DSM@ZJU-56 and Eu@ZnPZDDI and Eu@ZJU-56, were harvested and evaluated for use as potential ratiometric temperature probes.