Nanozyme-Based Colorimetric SARS-CoV-2 Nucleic Acid Detection by Naked Eye.

Fluorescence-based PCR and other amplification methods have been used for SARS-CoV-2 diagnostics, however, it requires costly fluorescence detectors and probes limiting deploying large-scale screening. Here, a cut-price colorimetric method for SARS-CoV-2 RNA detection by iron manganese silicate nanozyme (IMSN) is established. IMSN catalyzes the oxidation of chromogenic substrates by its peroxidase (POD)-like activity, which is effectively inhibited by pyrophosphate ions (PPi).

Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to Infection and Transfection.

The role of α-enolase (ENO1) in -related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. This study investigated the differential expression of α-enolase in clinical gastric specimens and cultured normal/cancer cells in response to () infection and transfection using qPCR, Western blots and histochemical methods. A total of 172 gastric specimens were collected from 142 patients, the former comprising chronic superficial gastritis (CSG), precancerous diseases (PCDs, including atrophic gastritis, intestinal metaplasia and dysplasia) and gastric cancer (GC) cases.

Molecular dynamics simulation-guided toehold mediated strand displacement probe for single-nucleotide variants detection.

Single nucleotide variant (SNV) has become an emerging biomarker for various diseases such as cancers and infectious diseases. Toehold-mediated strand displacement (TMSD), the core reaction of DNA nanotechnology, has been widely leveraged to identify SNVs. However, inappropriate choice of mismatch location results in poor discrimination ability.

Combination of parathyroid hormone pretreatment and mechanical stretch promotes osteogenesis of periodontal ligament fibroblasts.

Introduction: Parathyroid hormone (PTH) potentiates the mechanical loading induced bone formation in fracture healing and orthodontics. This study aimed to gain insight into the underlying mechanisms in periodontal ligament fibroblasts (PDLFs).

Methods: Human PDLFs were cultured and subjected to uniaxial cyclic stretch at 0.

Evaluation of the prognostic value of CBXs in gastric cancer patients.

Chromobox (CBX) proteins were suggested to exert epigenetic regulatory and transcriptionally repressing effects on target genes and might play key roles in the carcinogenesis of a variety of carcinomas. Nevertheless, the functions and prognostic significance of CBXs in gastric cancer (GC) remain unclear. The current study investigated the roles of CBXs in the prognosis of GC using the Oncomine, The Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN, The Cancer Genome Atlas (TCGA), and cBioPortal databases.

4-O-galloylalbiflorin inhibits the activity of CYP3A, 2C9, and 2D in human liver microsomes.

The effect of 4-O-galloylalbiflorin on the activity of cytochrome P450 enzymes (CYP450s) is an important factor that may induce drug-drug interaction.The effect of 4-O-galloylalbiflorin on the activity of CYP450s was evaluated in the presence of 0, 2.5, 5, 10, 25, 50, and 100 μM 4-O-galloylalbiflorin in pooled human liver microsomes.

MiRNA-214 promotes the pyroptosis and inhibits the proliferation of cervical cancer cells via regulating the expression of NLRP3.

Inflammasome mediates the maturation of interleukin-1β (IL-1β) and IL-18, triggers the pyroptosis and associates with multiple autoimmune diseases. In light of this, we hope to investigate the regulatory role of miRNA-214 in the inflammasome of cervical cancer. With the samples collected from 50 cervical cancer patients and 50 age-matched healthy subjects, real-time PCR and Western blotting were employed to detect the mRNA and/or protein expression profiles of the NOD-like receptor protein family, including NLRP1, NLRP3, NLRC4, Caspase-1, IL-1β, IL-18 and miR-214.

Dose-response association between C-reactive protein and risk of all-cause and cause-specific mortality: a systematic review and meta-analysis of cohort studies.

Purpose: The purpose of the study is to quantitatively assess the association between C-reactive protein (CRP) and all-cause, cardiovascular disease (CVD), and cancer mortality; a dose-response meta-analysis was performed on data from cohort studies in general population.

Methods: The published relevant articles were searched for in PubMed, Web of Science, and Embase until September 21, 2019. The pooled relative risk (RR) was estimated by random effects of generalized least square regression models.

Experimental study on Fenton oxidation regeneration of adsorbed toluene saturated activated carbon.

In this study, Fenton reagent has been adopted to oxidize and regenerate the resin based spherical activated carbon (SAC) adsorbed with toluene saturation. The effects of the mole ratio of Fe and HO, the dosage of HO, pH value, temperature, regeneration time and the like on the regeneration rate of activated carbon have been exploited, the adsorbent used in the experiment is spherical activated carbon with a maximum adsorption capacity of 512.25 mg/g produced by Cui hong Taiyuan Science and Technology Co.

Prognostic Significance of ADAM17 for Gastric Cancer Survival: A Meta-Analysis.

: The prognostic role of a disintegrin and metalloproteinase (ADAM) 17 has been widely assessed in gastric cancer. However, the results are inconsistent. We performed a meta-analysis to evaluate the prognostic significance of ADAM17 and its association with clinicopathological parameters.

[Expression of Gas6 in placenta and decidua tissues and its relationship with the pathogenesis of preeclampsia].

Objective: To investigate the expression of growth arrest-specific protein 6 (Gas6) in the placenta and decidua tissues and its relationship with the pathogenesis of preeclampsia.

Methods: All the patients were recruited in Qilu Hospital of Shangdong University from October 2013 to June 2014. Among them, thirty-two women with early-onset severe preeclampsia who received cesarean section were assigned to the preeclampsia group, and thirty healthy pregnant women who received cesarean section were defined as the control group.

[Expression of interleukin-37 in placenta and its relationship with the pathogenesis of severe preeclampsia].

Objective: To investigate the expression of interleukin-37 (IL-37) in placenta tissue and its relationship with the pathogenesis of severe preeclampsia.

Methods: All the patients were recruited in Qilu Hospital of Shangdong University from November 2012 to November 2013. Among them, thirty patients with severe preeclampsia were assigned to the preeclampsia group, and thirty-one healthy pregnant women were assigned to the control group.