UHPLC-QQQ-MS/MS method for the simultaneous quantification of 18 amino acids in various meats.

Amino acids are an essential source of human protein, and their content and composition are the main factors determining food protein utilization rate. Determining amino acids is essential in the component analysis of food. Therefore, a groundbreaking technique was developed utilizing ultra-high performance liquid chromatography interfaced with a triple quadrupole mass spectrometer (UHPLC-QQQ-MS/MS) for concurrently quantifying 18 amino acids across various types of meat.

ZntA maintains zinc and cadmium homeostasis and promotes oxidative stress resistance and virulence in .

Although metals are essential for life, they are toxic to bacteria in excessive amounts. Therefore, the maintenance of metal homeostasis is critical for bacterial physiology and pathogenesis. is a significant food-borne pathogen that mainly causes acute gastroenteritis in humans and acute hepatopancreatic necrosis disease in shrimp.

A modified shuffled frog leaping algorithm with inertia weight.

The shuffled frog leaping algorithm (SFLA) is a promising metaheuristic bionics algorithm, which has been designed by the shuffled complex evolution and the particle swarm optimization (PSO) framework. However, it is easily trapped into local optimum and has the low optimization accuracy when it is used to optimize complex engineering problems. To overcome the shortcomings, a novel modified shuffled frog leaping algorithm (MSFLA) with inertia weight is proposed in this paper.

Simultaneous determination of twenty-nine active compounds in by HPLC-QQQ-MS/MS.

As an empirical medicine of traditional Chinese medicine, have shown an effect against COVID-19 in clinical and inflammatory animal models. It is formulated with eight herbs, including , , , , , , , and . This study established a high-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QQQ-MS/MS) method by simultaneously determining 29 active compounds in the granules with significant content differences.

C-terminal domain of mammalian complexin-1 localizes to highly curved membranes.

In presynaptic nerve terminals, complexin regulates spontaneous "mini" neurotransmitter release and activates Ca-triggered synchronized neurotransmitter release. We studied the role of the C-terminal domain of mammalian complexin in these processes using single-particle optical imaging and electrophysiology. The C-terminal domain is important for regulating spontaneous release in neuronal cultures and suppressing Ca-independent fusion in vitro, but it is not essential for evoked release in neuronal cultures and in vitro.